Anti-Thymidylate Synthase antibody (ab168853)
Key features and details
- Rabbit polyclonal to Thymidylate Synthase
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Thymidylate Synthase antibody
See all Thymidylate Synthase primary antibodies -
Description
Rabbit polyclonal to Thymidylate Synthase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 263-313 of Human Thymidylate Synthase (NP_001062.1).
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Positive control
- WB: Jurkat, 293T, HeLa, mouse TCMK-1 and mouse NIH3T3 whole cell lysates. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab168853 was affinity purified using an epitope specific to Thymidylate Synthase immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 36 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
Pathway
Pyrimidine metabolism; dTTP biosynthesis. -
Sequence similarities
Belongs to the thymidylate synthase family. -
Cellular localization
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 740018 Chimpanzee
- Entrez Gene: 507631 Cow
- Entrez Gene: 607417 Dog
- Entrez Gene: 101148056 Gorilla
- Entrez Gene: 7298 Human
- Entrez Gene: 22171 Mouse
- Entrez Gene: 100459110 Orangutan
- Entrez Gene: 100627209 Pig
see all -
Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Images
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All lanes : Anti-Thymidylate Synthase antibody (ab168853) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds -
Detection of Thymidylate Synthase in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168853 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168853 has not yet been referenced specifically in any publications.