Key features and details
- Rabbit polyclonal to Thymidylate Synthase
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Thymidylate Synthase antibody
See all Thymidylate Synthase primary antibodies
DescriptionRabbit polyclonal to Thymidylate Synthase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
- Jurkat, 293T, HeLa, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168853 was affinity purified using an epitope specific to Thymidylate Synthase immobilized on solid support.
Our Abpromise guarantee covers the use of ab168853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 36 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
PathwayPyrimidine metabolism; dTTP biosynthesis.
Sequence similaritiesBelongs to the thymidylate synthase family.
Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
- Information by UniProt
- d TMP synthase antibody
- EC 220.127.116.11 antibody
- HsT422 antibody
All lanes : Anti-Thymidylate Synthase antibody (ab168853) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30 seconds
Detection of Thymidylate Synthase in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168853 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab168853 has not yet been referenced specifically in any publications.