Overview

  • Product name

    Anti-Thymidylate Synthase antibody
    See all Thymidylate Synthase primary antibodies
  • Description

    Rabbit polyclonal to Thymidylate Synthase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 263-313 of Human Thymidylate Synthase (NP_001062.1).

  • Positive control

    • Jurkat, 293T, HeLa, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-Thymidylate Synthase antibody (ab168853) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa


    Exposure time: 30 seconds
  • Detection of Thymidylate Synthase in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168853 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab168853 has not yet been referenced specifically in any publications.

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