Product nameAnti-Thymidylate Synthase antibody
See all Thymidylate Synthase primary antibodies
DescriptionSheep polyclonal to Thymidylate Synthase
Tested applicationsSuitable for: ELISA, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Escherichia coli, African green monkey
Does not react with: Saccharomyces cerevisiae
Recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
- HeLa cells treated with the TS inhibitor 5-FUdR
General notesThe antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab7398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.
Pre-clearing with a non-specific sheep IgG is helpful to reduce background.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.|
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
PathwayPyrimidine metabolism; dTTP biosynthesis.
Sequence similaritiesBelongs to the thymidylate synthase family.
Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
- Information by UniProt
- d TMP synthase antibody
- EC 220.127.116.11 antibody
- HsT422 antibody
ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.
IHC image of Thymidylate Synthase staining in Human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7398, 5µg/ml, for 15 mins at room temperature. A Rabbit anti-Sheep biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Gu YJ et al. GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma. Oncotarget 6:33658-74 (2015). WB . Read more (PubMed: 26378040) »
- MacFarlane AJ et al. Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity. J Nutr 144:419-24 (2014). Read more (PubMed: 24500934) »