Recombinant Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-298901-anti-thymidylate-synthase-antibody-epr4545-western-blot.jpg "Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4545] to Thymidylate Synthase
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Thymidylate Synthase antibody [EPR4545]
See all Thymidylate Synthase primary antibodies -
Description
Rabbit monoclonal [EPR4545] to Thymidylate Synthase -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat, HL60 and MCF7 cell lysates; Human ovary carcinoma and colon carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4545 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Thymidylate Synthase antibody [EPR4545] (ab197898)
- Alexa Fluor® 647 Anti-Thymidylate Synthase antibody [EPR4545] (ab197899)
- APC Anti-Thymidylate Synthase antibody [EPR4545] (ab225091)
- PE Anti-Thymidylate Synthase antibody [EPR4545] (ab225092)
- Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (ab232021)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
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| IP |
1/10 - 1/100.
|
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| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
Pathway
Pyrimidine metabolism; dTTP biosynthesis. -
Sequence similarities
Belongs to the thymidylate synthase family. -
Cellular localization
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7298 Human
- Entrez Gene: 22171 Mouse
- Entrez Gene: 29261 Rat
- Omim: 188350 Human
- SwissProt: P04818 Human
- SwissProt: P07607 Mouse
- SwissProt: P45352 Rat
- Unigene: 369762 Human
see all -
Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Images
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Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/10000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-298866-anti-thymidylate-synthase-antibody-epr4545-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)ab108995 (purified) at 1:30 dilution (2μg) immunoprecipitating Thymidylate Synthase in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate,10μg
Lane 2 (+): ab108995 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108995 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-298511-anti-thymidylate-synthase-antibody-epr4545-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Thymidylate Synthase with purified ab108995 at 1/50 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-298496-anti-thymidylate-synthase-antibody-epr4545-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Thymidylate Synthas with Purified ab108995 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100 ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-298357-anti-thymidylate-synthase-antibody-epr4545-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Thymidylate Synthase with purified ab108995 at 1:100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-121531-anti-thymidylate-synthase-antibody-epr4545-western-blot.jpg)
Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)All lanes : Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-121530-anti-thymidylate-synthase-antibody-epr4545-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)Unpurified ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)](https://www.abcam.com/ps/products/108/ab108995/Images/ab108995-8-benefits-of-recombinant-antibodies.png)
Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab108995 has been referenced in 12 publications.
- Wang D et al. Tumor mutation burden as a biomarker in resected gastric cancer via its association with immune infiltration and hypoxia. Gastric Cancer 24:823-834 (2021). PubMed: 33687617
- Shorstova T et al. Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition. Mol Cancer Ther 20:64-75 (2021). PubMed: 33087508
- Tao Q et al. Perineural Invasion and Postoperative Adjuvant Chemotherapy Efficacy in Patients With Gastric Cancer. Front Oncol 10:530 (2020). PubMed: 32373527
- Zhang S et al. Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence. Oncol Rep 44:565-576 (2020). PubMed: 32627015
- Duan R et al. Tumor Microenvironment Status Predicts the Efficacy of Postoperative Chemotherapy or Radiochemotherapy in Resected Gastric Cancer. Front Immunol 11:609337 (2020). PubMed: 33569057