Product nameAnti-Thymidylate Synthase antibody [EPR4545] (Phycoerythrin)
See all Thymidylate Synthase primary antibodies
DescriptionRabbit monoclonal [EPR4545] to Thymidylate Synthase (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Thymidylate Synthase aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P04818
- Flow Cyt: HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
- Anti-Thymidylate Synthase antibody [EPR4545] (Alexa Fluor® 488) (ab197898)
- Anti-Thymidylate Synthase antibody [EPR4545] (Alexa Fluor® 647) (ab197899)
- Anti-Thymidylate Synthase antibody [EPR4545] (Allophycocyanin) (ab225091)
- Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (ab232021)
Our Abpromise guarantee covers the use of ab225092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
PathwayPyrimidine metabolism; dTTP biosynthesis.
Sequence similaritiesBelongs to the thymidylate synthase family.
Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
- Information by UniProt
- d TMP synthase antibody
- EC 18.104.22.168 antibody
- HsT422 antibody
Overlay histogram showing HeLa cells stained with ab225092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225092, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab225092 has not yet been referenced specifically in any publications.