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Recombinant Anti-Thymidylate Synthase antibody [SP112] (ab111153)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
  • Flow Cytometry - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
  • Anti-Thymidylate Synthase antibody [SP112] (ab111153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP112] to Thymidylate Synthase
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human Thymidylate Synthase protein (ab95378)

View more associated products

Overview

  • Product name

    Anti-Thymidylate Synthase antibody [SP112]
    See all Thymidylate Synthase primary antibodies
  • Description

    Rabbit monoclonal [SP112] to Thymidylate Synthase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Colon carcinoma and tonsil tissue, human hepatocellular carcinoma. Flow Cyt: HeLa cells.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP112
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Purine and pyrimidine synthesis

Associated products

  • Alternative Versions

    • Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (ab240947)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Thymidylate Synthase protein (ab95378)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab111153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

Flow Cyt
1/200.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

Flow Cyt
1/200.

Target

  • Function

    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • Pathway

    Pyrimidine metabolism; dTTP biosynthesis.
  • Sequence similarities

    Belongs to the thymidylate synthase family.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Target information above from: UniProt accession P04818 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7298 Human
    • Omim: 188350 Human
    • SwissProt: P04818 Human
    • Unigene: 369762 Human
    • Alternative names

      • d TMP synthase antibody
      • EC 2.1.1.45 antibody
      • HsT422 antibody
      • MGC88736 antibody
      • OTTHUMP00000162195 antibody
      • Thymidylate synthase antibody
      • Thymidylate synthetase antibody
      • TMS antibody
      • TS antibody
      • TSase antibody
      • Tyms antibody
      • TYMS protein antibody
      • Tyms thymidylate synthetase antibody
      • TYSY_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human hepatocellular carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab111153 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, with some nuclear staining, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab111153 for 30 mins at room temperature.

    • Flow Cytometry - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      Flow Cytometry - Anti-Thymidylate Synthase antibody [SP112] (ab111153)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Thymidylate Synthase with purified ab111153 at 1/200 dilution (0.78 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      ab111153 at 1/100 dilution staining Thymidylate Synthase in Formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue.
    • Anti-Thymidylate Synthase antibody [SP112] (ab111153)
      Anti-Thymidylate Synthase antibody [SP112] (ab111153)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab111153? Please let us know so that we can cite the reference in this datasheet.

    ab111153 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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