Recombinant
RabMAb

Recombinant Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (ab240947)

Overview

  • Product name

    Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free
    See all Thymidylate Synthase primary antibodies
  • Description

    Rabbit monoclonal [SP112] to Thymidylate Synthase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thymidylate Synthase aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P04818

  • Positive control

    • Colon carcinoma and tonsil tissue, Human hepatocellular carcinoma
  • General notes

    Ab240947 is the carrier-free version of ab111153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240947 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab240947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

 

Target

  • Function

    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • Pathway

    Pyrimidine metabolism; dTTP biosynthesis.
  • Sequence similarities

    Belongs to the thymidylate synthase family.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • d TMP synthase antibody
    • EC 2.1.1.45 antibody
    • HsT422 antibody
    • MGC88736 antibody
    • OTTHUMP00000162195 antibody
    • Thymidylate synthase antibody
    • Thymidylate synthetase antibody
    • TMS antibody
    • TS antibody
    • TSase antibody
    • Tyms antibody
    • TYMS protein antibody
    • Tyms thymidylate synthetase antibody
    • TYSY_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human hepatocellular carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab111153 for 30 mins at room temperature.

    This image was generated using ab111153, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, with some nuclear staining, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab111153 for 30 mins at room temperature.

    This image was generated using ab111153, the same clone, but with a different buffer formulation.

  • ab111153 at 1/100 dilution staining Thymidylate Synthase in Formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111153).

References

ab240947 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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