Key features and details
- Mouse monoclonal [TS106 + TMS715] to Thymidylate Synthase - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Thymidylate Synthase antibody [TS106 + TMS715] - BSA and Azide free
See all Thymidylate Synthase primary antibodies
DescriptionMouse monoclonal [TS106 + TMS715] to Thymidylate Synthase - BSA and Azide free
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, IP, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Thymidylate Synthase aa 1-313.
MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDR TGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKE LSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDME SDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQ FYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIH TLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEG YNPHPTIKMEMAV
Database link: P04818
- IHC-P: Human testicular carcinoma tissue.
ab269736 is a carrier free version of ab199003. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. Both antibody clones TS1 6 + TMS715 are mouse IgG1 kappa
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Clone numberTS106 + TMS715
Light chain typekappa
Our Abpromise guarantee covers the use of ab269736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 10 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 1-2 µg/mg of lysate.|
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
PathwayPyrimidine metabolism; dTTP biosynthesis.
Sequence similaritiesBelongs to the thymidylate synthase family.
Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
- Information by UniProt
- d TMP synthase antibody
- EC 22.214.171.124 antibody
- HsT422 antibody
Formalin-fixed, paraffin-embedded human Testicular Carcinoma tissue stained for thymidylate Synthase using ab199003 at 2 µg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269736).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269736 has not yet been referenced specifically in any publications.