Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)
Key features and details
- Mouse monoclonal [TYMS/1884] to Thymidylate Synthase
- Suitable for: Protein Array, IHC-P, ICC, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Thymidylate Synthase antibody [TYMS/1884]
See all Thymidylate Synthase primary antibodies -
Description
Mouse monoclonal [TYMS/1884] to Thymidylate Synthase -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Thymidylate Synthase aa 60-174. The exact sequence is proprietary.
Database link: P04818 -
Positive control
- IHC-P: Human tonsil tissue; ICC: Ramos cells; Flow Cyt: MOLT-4 cells; WB: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab224649 was purified from Bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
TYMS/1884 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt |
Use 1-2µg for 106 cells.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use 1-2µg for 106 cells. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
Pathway
Pyrimidine metabolism; dTTP biosynthesis. -
Sequence similarities
Belongs to the thymidylate synthase family. -
Cellular localization
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7298 Human
- Omim: 188350 Human
- SwissProt: P04818 Human
- Unigene: 369762 Human
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Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Images
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Flow Cytometry analysis of PFA-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling Thymidylate Synthase with ab224649 at 2 µg/106 cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Flow Cytometry analysis of PFA-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling Thymidylate Synthase with ab224649 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).
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Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 36 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Thymidylate Synthase using ab224649 at 2 µg/ml in immunohistochemical analysis.
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ab224649 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224649 has not yet been referenced specifically in any publications.