Overview

  • Product name

    Anti-Thymidylate Synthase antibody [TYMS/1884]
    See all Thymidylate Synthase primary antibodies
  • Description

    Mouse monoclonal [TYMS/1884] to Thymidylate Synthase
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Thymidylate Synthase aa 60-174. The exact sequence is proprietary.
    Database link: P04818

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab224649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • Pathway

    Pyrimidine metabolism; dTTP biosynthesis.
  • Sequence similarities

    Belongs to the thymidylate synthase family.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • d TMP synthase antibody
    • EC 2.1.1.45 antibody
    • HsT422 antibody
    • MGC88736 antibody
    • OTTHUMP00000162195 antibody
    • Thymidylate synthase antibody
    • Thymidylate synthetase antibody
    • TMS antibody
    • TS antibody
    • TSase antibody
    • Tyms antibody
    • TYMS protein antibody
    • Tyms thymidylate synthetase antibody
    • TYSY_HUMAN antibody
    see all

Images

  • ab224649 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Thymidylate Synthase using ab224649 at 2 µg/ml in immunohistochemical analysis.

References

ab224649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224649.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up