Product nameThymidylate Synthase overexpression 293T lysate (whole cell)
DescriptionThymidylate Synthase 293T Transfected Lysate - (positive control)
ab94158 is a 293T cell transfected lysate in which Human Thymidylate Synthase has been transiently over-expressed using a pCMV-Thymidylate Synthase plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Pathway: Pyrimidine metabolism; dTTP biosynthesis. Similarity: Belongs to the thymidylate synthase family.
Our Abpromise guarantee covers the use of ab94158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94158 at 15 µg/lane on an SDS-PAGE gel
All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1/500 dilution
Lane 1 :
Thymidylate Synthase overexpression 293T lysate (whole cell) (ab94158)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94158 has not yet been referenced specifically in any publications.