Anti-Thymine Dimer antibody [H3] (ab10347)
![Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)](https://www.abcam.com/ps/products/10/ab10347/Images/ab10347-17951-anti-thymine-dimer-antibody-h3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)")
Key features and details
- Mouse monoclonal [H3] to Thymine Dimer
- Suitable for: Southern Blot, ICC, Competitive ELISA, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Thymine Dimer antibody [H3] -
Description
Mouse monoclonal [H3] to Thymine Dimer -
Host species
Mouse -
Tested applications
Suitable for: Southern Blot, ICC, Competitive ELISA, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule.
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Positive control
- ICC/IF: HeLa cells.
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General notes
Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis. -
Clonality
Monoclonal -
Clone number
H3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Southern Blot |
Use a concentration of 0.5 - 1 µg/ml.
|
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| ICC |
Use at an assay dependent dilution.
|
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| Competitive ELISA |
Use at an assay dependent dilution.
|
|
| ELISA | (1) |
Use at an assay dependent dilution.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Southern Blot
Use a concentration of 0.5 - 1 µg/ml. |
|
ICC
Use at an assay dependent dilution. |
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Competitive ELISA
Use at an assay dependent dilution. |
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ELISA
Use at an assay dependent dilution. |
|
ICC/IF
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thymine Dimer antibody [H3] (ab10347)This image is courtesy of an anonymous Abreview.ab10347 staining Thymine Dimer in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in formaldehyde, permabilized using 0.5% Triton X-100, blocked with 5% BSA for 15 minutes at 20°C, then incubated with ab10347 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated rabbit anti mouse polyclonal, used at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab10347 has been referenced in 10 publications.
- Tedeschi F et al. EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis. New Phytol 221:261-278 (2019). PubMed: 30252137
- Yadav VK et al. Detection of UV-Induced Thymine Dimers. Methods Mol Biol 2031:313-322 (2019). PubMed: 31473968
- Cobb AM et al. Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling. Nucleus 7:498-511 (2016). PubMed: 27676213
- Torregrosa-Muñumer R et al. Low doses of ultraviolet radiation and oxidative damage induce dramatic accumulation of mitochondrial DNA replication intermediates, fork regression, and replication initiation shift. Mol Biol Cell 26:4197-208 (2015). PubMed: 26399294
- Deng Y et al. A sunblock based on bioadhesive nanoparticles. Nat Mater 14:1278-85 (2015). PubMed: 26413985
- Anda S et al. Cell-cycle analyses using thymidine analogues in fission yeast. PLoS One 9:e88629 (2014). PubMed: 24551125
- Dantas TJ et al. Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins. J Cell Biol 193:307-18 (2011). WB . PubMed: 21482720
- Myakishev-Rempel M et al. Investigation of the peak action wavelength of light-activated gene transduction. Gene Ther 18:1043-51 (2011). PubMed: 21490685
- Moriel-Carretero M & Aguilera A A Postincision-Deficient TFIIH Causes Replication Fork Breakage and Uncovers Alternative Rad51- or Pol32-Mediated Restart Mechanisms. Mol Cell 37:690-701 (2010). PubMed: 20227372
- Parro V et al. SOLID2: an antibody array-based life-detector instrument in a Mars Drilling Simulation Experiment (MARTE). Astrobiology 8:987-99 (2008). ELISA . PubMed: 19105755