Overview

  • Product name
    Anti-Thymosin beta 4 antibody
    See all Thymosin beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Thymosin beta 4
  • Host species
    Rabbit
  • Specificity
    Recognises a synthetic Human Thymosin beta 4 peptide(aa 1-43). The antibody has not been tested on the endogenous protein.ab14334 also detects Thymosin beta 9 and Thymosin beta 10 along with Thymosin beta 4.
  • Tested applications
    Suitable for: ELISA, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow
  • Immunogen

    Synthetic peptide: SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES conjugated to KLH, corresponding to amino acids 1-43 of Human Thymosin beta 4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Thymosin beta 4 synthetic peptide

Properties

Applications

Our Abpromise guarantee covers the use of ab14334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
ICC 1/100.
IHC-P 1/100 - 1/1000.
WB Use at an assay dependent concentration. See Smart et al. Non specific bands were reported by the researcher who published this reference.

Target

References

This product has been referenced in:
  • Kannan L  et al. Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF. Ann N Y Acad Sci 1112:425-34 (2007). Read more (PubMed: 17947593) »
  • Smart N  et al. Thymosin beta4 induces adult epicardial progenitor mobilization and neovascularization. Nature : (2006). Read more (PubMed: 17108969) »

See all 2 Publications for this product

Publishing research using ab14334? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Thank you for your reply. I have completed the questionnaire and have also attached pictures. I would be grateful if you could let me know if there are any differences in concentration/activity between the two lots.

Many thanks

<...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.


Reviewing this case, I...

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Inquiry: Hello, We have been using this antibody for IHC on paraffin embedded samples and was working fine with the previous lot (944043). However, we have been experiencing problems with the new lot we have recently received (GR2453-5). Instead of get...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this lot of ab14334 Thymosin beta 4 antibody.

I would like to reassure you that we monitor feedback closely on a week...

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Dear tech,

A customer notices that the color of ab14334(lot GR2453-2 in order 1077613) is abnormal. Would you please check attached pictures? Thank you for your help!

Best regards,
Zhang Yingdan

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Thank you for contacting us.

ab14334 is whole serum so it is normal to be brown in colour.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use o...

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According to your data sheet this product has been used in ELISA. Do you have any additional information concerning a suggested protocol or further details that can be used with this application? Thank You.

Thank you for your enquiry. I have been able to source the protocol for direct ELISA which was used with ab14334 and have added it to the datasheet for your convenience. The antibody was tested against the immunizing peptide (Thymosin ß4 synthetic pep...

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I was curious whether you have any information now concerning the use of this antibody for Western blotting, immunohistochemistry, or with mouse thymosin. Additionally, do you ship the product with a positive control, or could you recommend an appropr...

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Thank you for your enquiry regarding ab14334. I can confirm that the antibody has currently only been tested in ELISA on the human synthetic peptide used to generate this antibody. We do not currently supply this peptide or a positive control and I...

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Western blot abreview for Anti-Thymosin beta 4 antibody

 Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Aug 09 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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