Overview

  • Product name
    Anti-Thymosin beta 4 antibody
    See all Thymosin beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Thymosin beta 4
  • Host species
    Rabbit
  • Specificity
    Human Thymosin B4 (aa 1-11)
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human Thymosin beta 4 aa 1-11 conjugated to keyhole limpet haemocyanin.
    Sequence:

    SDKPDMAEIEK


    Database link: P62328

  • Positive control
    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab14335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF 1/200.
IHC-P 1/100 - 1/1000.

Tested in lung and heart tissues.

Target

  • Function
    Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
    Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase.
  • Tissue specificity
    Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
  • Sequence similarities
    Belongs to the thymosin beta family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fx antibody
    • Hematopoietic system regulatory peptide antibody
    • Prothymosin beta 4 antibody
    • PTMB 4 antibody
    • PTMB4 antibody
    • Seraspenide antibody
    • T beta 4 antibody
    • T beta-4 antibody
    • TB4X antibody
    • THYB 4 antibody
    • Thyb4 antibody
    • Thymosin beta 4 antibody
    • Thymosin beta 4 X chromosome antibody
    • Thymosin beta 4 X linked antibody
    • TMSB 4 antibody
    • TMSB4 antibody
    • Tmsb4x antibody
    • TYB4_HUMAN antibody
    see all

Images

  • ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.  

  • Indirect ELISA analysis. Anti-Thymosin beta 4 antibody (ab14335) at 1/1000 dilution. Sample: chicken heart culture serum. An alkaline phosphatase conjugated donkey anti-rabbit secondary antibody was used at 1/8000

    See Abreview

References

This product has been referenced in:
  • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »
  • Fu X  et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . Read more (PubMed: 25695679) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Xenopus laevis Cell (Brain)
Specification
Brain
Permeabilization
Yes - 0.01% Triton X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 27 2014

Application
ELISA
Sample
Chicken Serum (heart culture)
Specification
heart culture
Type
Indirect
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Yanyang Li

Verified customer

Submitted Jan 13 2014

Answer

DISCOUNT CODE: xxxxxxx
Expiration date: 10th May 2013
Value: £235

I am pleased to hear you would like to participate in the AbTrial scheme. As discussed, I have generated the discount code above. This code will give you £235 off your next order before the expiration date. Please note, the antibody ab14335 must be purchased full price as normal, an existing AbTrial code cannot be used.

To redeem this offer, please submit an Abreview for Xenopus in Western blotting or ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

Read More

Answer


for ab101672:
Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
Positive control: hepatocarcinoma .


for ab14335:
No antigen retrieval is necessary, the protocol is as follows:
1. Mount 8 µm paraffin sections on slides
2. Deparaffinize
3. Wash 4x 10 min with PBS
4. Dry slide around sections, encircle sections with pap pen
5. Incubate sections with 10% normal goat serum in a moist chamber
6. Remove normal serum
7. Apply antibody diluted 1:1000 in PBS-BSA
8. Incubate 24-48 hours at 4°C in moist chamber
9. Wash 4x 10 min with PBS
10. Incubate for 2 hours with secondary fluorescent-labeled antibody in PBS-BSA
11. Wash 2x 10 min with PBS
12. Stain with DAPI
13. Wash, dry and mount sections in mounting medium
Positive control: Basically, Thymosin beta 4 is expressed in all organs and tissues. We have used human heart, liver, lung and brain so far.

Read More

Answer

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab121520, the datasheet states "Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol."

For the other 2 antibodies I have contacted the lab to find out what is recommended. I will let you know.

Could you please describe to me which problem you had with each antibody as well as protocol information and what tissue samples were used, that would be of great help to look into this in greater detail.

I look forward to hear back from you and assist you further.

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