Anti-Thymosin beta 4 antibody (ab14335)

DISCOUNT CODE: xxxxxxx
Expiration date: 10th May 2013
Value: £235

I am pleased to hear you would like to participate in the AbTrial scheme. As discussed, I have generated the discount code above. This code will give you £235 off your next order before the expiration date. Please note, the antibody ab14335 must be purchased full price as normal, an existing AbTrial code cannot be used.

To redeem this offer, please submit an Abreview for Xenopus in Western blotting or ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

for ab101672:
Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
Positive control: hepatocarcinoma .


for ab14335:
No antigen retrieval is necessary, the protocol is as follows:
1. Mount 8 µm paraffin sections on slides
2. Deparaffinize
3. Wash 4x 10 min with PBS
4. Dry slide around sections, encircle sections with pap pen
5. Incubate sections with 10% normal goat serum in a moist chamber
6. Remove normal serum
7. Apply antibody diluted 1:1000 in PBS-BSA
8. Incubate 24-48 hours at 4°C in moist chamber
9. Wash 4x 10 min with PBS
10. Incubate for 2 hours with secondary fluorescent-labeled antibody in PBS-BSA
11. Wash 2x 10 min with PBS
12. Stain with DAPI
13. Wash, dry and mount sections in mounting medium
Positive control: Basically, Thymosin beta 4 is expressed in all organs and tissues. We have used human heart, liver, lung and brain so far.

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab121520, the datasheet states "Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol."

For the other 2 antibodies I have contacted the lab to find out what is recommended. I will let you know.

Could you please describe to me which problem you had with each antibody as well as protocol information and what tissue samples were used, that would be of great help to look into this in greater detail.

I look forward to hear back from you and assist you further.