- Datasheet
- Specific References (3)
- Protocols
Overview
-
Product nameAnti-Thymosin beta 4 antibody
See all Thymosin beta 4 primary antibodies -
DescriptionRabbit polyclonal to Thymosin beta 4
-
Host speciesRabbit
-
SpecificityHuman Thymosin B4 (aa 1-11)
-
Tested applicationsSuitable for: ELISA, ICC/IF, IHC-Pmore details
-
Species reactivityReacts with: Rat, Chicken, Human
-
Immunogen
Synthetic peptide corresponding to Human Thymosin beta 4 aa 1-11 conjugated to keyhole limpet haemocyanin.
Sequence:SDKPDMAEIEK
Database link: P62328 -
Positive control
- ICC/IF: HeLa cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
-
PurityWhole antiserum
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab14335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000. | |
| ICC/IF | 1/200. | |
| IHC-P | 1/100 - 1/1000. Tested in lung and heart tissues. |
Target
-
FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase. -
Tissue specificityExpressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
-
Sequence similaritiesBelongs to the thymosin beta family.
-
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
-
Database links
- Entrez Gene: 7114 Human
- Entrez Gene: 81814 Rat
- Omim: 300159 Human
- SwissProt: P62328 Human
- SwissProt: P62329 Rat
- Unigene: 437277 Human
- Unigene: 168067 Rat
- Unigene: 2605 Rat
-
Alternative names
- Fx antibody
- Hematopoietic system regulatory peptide antibody
- Prothymosin beta 4 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Thymosin beta 4 antibody (ab14335)ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)Image from Lagarrigue M et al., Mol Cell Proteomics, 10, M110.005991. Fig 6.; doi: 10.1074/mcp.M110.005991.Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.
-
ELISA - Anti-Thymosin beta 4 antibody (ab14335)This image is courtesy of an abreview submitted by Yanyang Li, University of IllinoisIndirect ELISA analysis. Anti-Thymosin beta 4 antibody (ab14335) at 1/1000 dilution. Sample: chicken heart culture serum. An alkaline phosphatase conjugated donkey anti-rabbit secondary antibody was used at 1/8000
References
This product has been referenced in:
- Wong SW et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). Read more (PubMed: 25371376) »
- Fu X et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . Read more (PubMed: 25695679) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"