For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    thymosin-beta-4-antibody-ab14335.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
Share by email

Anti-Thymosin beta 4 antibody (ab14335)

  • Datasheet
Reviews (2)Q&A (3)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)

Key features and details

  • Rabbit polyclonal to Thymosin beta 4
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Thymosin beta 4 protein (ab112365)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human TMSB4X (Thymosin beta 4) knockout HEK-293T cell line (ab266490)

View more associated products

Overview

  • Product name

    Anti-Thymosin beta 4 antibody
    See all Thymosin beta 4 primary antibodies
  • Description

    Rabbit polyclonal to Thymosin beta 4
  • Host species

    Rabbit
  • Specificity

    Human Thymosin B4 (aa 1-11)
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Thymosin beta 4 aa 1-11 conjugated to keyhole limpet haemocyanin.
    Sequence:

    SDKPDMAEIEK


    Database link: P62328
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Thymosin beta 4 protein (ab112365)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/1000.

Tested in lung and heart tissues.

Notes
IHC-P
1/100 - 1/1000.

Tested in lung and heart tissues.

Target

  • Function

    Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
    Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase.
  • Tissue specificity

    Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.
  • Sequence similarities

    Belongs to the thymosin beta family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62328 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7114 Human
    • Omim: 300159 Human
    • SwissProt: P62328 Human
    • Unigene: 437277 Human
    • Alternative names

      • Fx antibody
      • Hematopoietic system regulatory peptide antibody
      • Prothymosin beta 4 antibody
      • PTMB 4 antibody
      • PTMB4 antibody
      • Seraspenide antibody
      • T beta 4 antibody
      • T beta-4 antibody
      • TB4X antibody
      • THYB 4 antibody
      • Thyb4 antibody
      • Thymosin beta 4 antibody
      • Thymosin beta 4 X chromosome antibody
      • Thymosin beta 4 X linked antibody
      • TMSB 4 antibody
      • TMSB4 antibody
      • Tmsb4x antibody
      • TYB4_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)Image from Lagarrigue M et al., Mol Cell Proteomics, 10, M110.005991. Fig 6.; doi: 10.1074/mcp.M110.005991.

      Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymosin beta 4 antibody (ab14335)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hear tissue sections labeling Thymosin beta 4 with ab14335 at 1/100 dilution, followed by an appropriate secondary antibody conjugated to Cy3. The nuclei were stained with DAPI.  

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (4)

    Publishing research using ab14335? Please let us know so that we can cite the reference in this datasheet.

    ab14335 has been referenced in 4 publications.

    • Mantri M  et al. Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis. Nat Commun 12:1771 (2021). PubMed: 33741943
    • Wong SW  et al. H2O 2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization. Ann Biomed Eng 43:1178-88 (2015). PubMed: 25371376
    • Fu X  et al. Thymosin ß4 promotes hepatoblastoma metastasis via the induction of epithelial-mesenchymal transition. Mol Med Rep 12:127-32 (2015). IHC ; Human . PubMed: 25695679
    • Lagarrigue M  et al. Revisiting Rat Spermatogenesis with MALDI Imaging at 20-{micro}m Resolution. Mol Cell Proteomics 10:M110.005991 (2011). IHC-P ; Rat . PubMed: 21149303

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Thymosin beta 4 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample
    Xenopus laevis Cell (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.01% Triton X-100
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

    ELISA abreview for Anti-Thymosin beta 4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Chicken Serum (heart culture)
    Specification
    heart culture
    Type
    Indirect
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Yanyang Li

    Verified customer

    Submitted Jan 13 2014

    Question

    Phone call requesting AbTrial discount for ab14335 for use with Xenopus samples in ICC and WB.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 08 2013

    Answer

    DISCOUNT CODE: xxxxxxx
    Expiration date: 10th May 2013
    Value: £235

    I am pleased to hear you would like to participate in the AbTrial scheme. As discussed, I have generated the discount code above. This code will give you £235 off your next order before the expiration date. Please note, the antibody ab14335 must be purchased full price as normal, an existing AbTrial code cannot be used.

    To redeem this offer, please submit an Abreview for Xenopus in Western blotting or ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site:

    www.abcam.com/Abreviews

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here:

    www.abcam.com/abtrial

    Read More

    Abcam Scientific Support

    Answered on Jan 08 2013

    Question

    IHC-P with human tissue
    used citrate buffer for AR
    no signal

    - which antigen retrieval method recommended?
    - which positive control to be used?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2012

    Answer


    for ab101672:
    Antigen retrieval: Sodium citrate buffer (10 mM sodium citrate, pH 6.0), boil for 15 minutes.
    Positive control: hepatocarcinoma .


    for ab14335:
    No antigen retrieval is necessary, the protocol is as follows:
    1. Mount 8 µm paraffin sections on slides
    2. Deparaffinize
    3. Wash 4x 10 min with PBS
    4. Dry slide around sections, encircle sections with pap pen
    5. Incubate sections with 10% normal goat serum in a moist chamber
    6. Remove normal serum
    7. Apply antibody diluted 1:1000 in PBS-BSA
    8. Incubate 24-48 hours at 4°C in moist chamber
    9. Wash 4x 10 min with PBS
    10. Incubate for 2 hours with secondary fluorescent-labeled antibody in PBS-BSA
    11. Wash 2x 10 min with PBS
    12. Stain with DAPI
    13. Wash, dry and mount sections in mounting medium
    Positive control: Basically, Thymosin beta 4 is expressed in all organs and tissues. We have used human heart, liver, lung and brain so far.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2012

    Question

    IHC-P with human tissue
    used citrate buffer for AR
    no signal

    - which antigen retrieval method recommended?
    - which positive control to be used?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    Thank you for contacting us.

    For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

    For ab121520, the datasheet states "Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol."

    For the other 2 antibodies I have contacted the lab to find out what is recommended. I will let you know.

    Could you please describe to me which problem you had with each antibody as well as protocol information and what tissue samples were used, that would be of great help to look into this in greater detail.

    I look forward to hear back from you and assist you further.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.