Anti-Thymosin beta 4 antibody (ab167650)
Key features and details
- Mouse polyclonal to Thymosin beta 4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thymosin beta 4 antibody
See all Thymosin beta 4 primary antibodies -
Description
Mouse polyclonal to Thymosin beta 4 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig -
Immunogen
Full length protein, corresponding to amino acids 1-44 of Human Thymosin beta 4 (AAH01631.1).
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Positive control
- Thymosin beta 4 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 5 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 5 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase. -
Tissue specificity
Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells. -
Sequence similarities
Belongs to the thymosin beta family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 282386 Cow
- Entrez Gene: 100034015 Horse
- Entrez Gene: 7114 Human
- Entrez Gene: 19241 Mouse
- Entrez Gene: 733606 Pig
- Entrez Gene: 81814 Rat
- Omim: 300159 Human
- SwissProt: P62327 Horse
see all -
Alternative names
- Fx antibody
- Hematopoietic system regulatory peptide antibody
- Prothymosin beta 4 antibody
see all
Images
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All lanes : Anti-Thymosin beta 4 antibody (ab167650) at 1 µg/ml
Lane 1 : Thymosin beta 4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 5 kDa -
Immunofluorescent analysis of HeLa cells labeling Thymosin beta 4 with ab167650 at 10 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab167650 has been referenced in 3 publications.
- Wang F et al. Thymosin β4 Protects against Cardiac Damage and Subsequent Cardiac Fibrosis in Mice with Myocardial Infarction. Cardiovasc Ther 2022:1308651 (2022). PubMed: 35712678
- Wang M et al. Thymosin ß4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-?B signaling axis in APP/PS1 mice. J Neuroinflammation 18:146 (2021). PubMed: 34183019
- Wang M et al. 5-HT1AR alleviates Aß-induced cognitive decline and neuroinflammation through crosstalk with NF-?B pathway in mice. Int Immunopharmacol 82:106354 (2020). PubMed: 32143008