Anti-Thyroglobulin antibody [2H11] - BSA and Azide free (ab212867)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [2H11] - BSA and Azide free (ab212867)](https://www.abcam.com/ps/products/212/ab212867/Images/ab212867-264618-ab212867.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [2H11] - BSA and Azide free (ab212867)")
Key features and details
- Mouse monoclonal [2H11] to Thyroglobulin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Thyroglobulin antibody [2H11] - BSA and Azide free
See all Thyroglobulin primary antibodies -
Description
Mouse monoclonal [2H11] to Thyroglobulin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Human Thyroglobulin. (Human thyroid follicular cells).
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Positive control
- Human thyroid tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab212867 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
2H11 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab212867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.1 - 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 min at RT). |
Target
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Function
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Tissue specificity
Thyroid gland specific. -
Involvement in disease
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
Post-translational
modificationsSulfated tyrosines are desulfated during iodination. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7038 Human
- Entrez Gene: 21819 Mouse
- Entrez Gene: 24826 Rat
- Omim: 188450 Human
- SwissProt: P01266 Human
- SwissProt: O08710 Mouse
- SwissProt: P06882 Rat
- Unigene: 654591 Human
see all -
Alternative names
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [2H11] - BSA and Azide free (ab212867)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid tissue labeling Thyroglobulin with ab212867 at 0.2 µg/ml.
Datasheets and documents
References (0)
ab212867 has not yet been referenced specifically in any publications.
