RecombinantRabMAb

Recombinant Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

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ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
  • Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
  • Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CPT-R35.3-41] to Thyroglobulin
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-Thyroglobulin antibody [CPT-R35.3-41]
    See all Thyroglobulin primary antibodies

  • Description

    Rabbit monoclonal [CPT-R35.3-41] to Thyroglobulin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human thyroid tissue lysate. ELISA: Immunizing peptide.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CPT-R35.3-41

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 330 kDa (predicted molecular weight: 305 kDa).
ELISA
Use a concentration of 0.015 - 1 µg/ml.
Notes
WB
1/1000. Detects a band of approximately 330 kDa (predicted molecular weight: 305 kDa).
ELISA
Use a concentration of 0.015 - 1 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

    • Tissue specificity

      Thyroid gland specific.

    • Involvement in disease

      Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
      Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.

    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.
      Contains 11 thyroglobulin type-1 domains.

    • Post-translational
      modifications

      Sulfated tyrosines are desulfated during iodination.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • AITD 3 antibody
        • AITD3 antibody
        • hTG antibody
        • TDH3 antibody
        • Tg antibody
        • TGN antibody
        • THYG_HUMAN antibody
        • Thyroglobulin antibody
        see all

      Images

      • ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
        ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

        50ng CPTAC-39d (1000ng/mL, 50 μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243094 (50μL) were incubated with CPTAC-39d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

        Secondary antibody was used at 1/2500 dilution.

      • Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
        Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
        All lanes : Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094) at 1/1000 dilution

        Lane 1 : Human kidney tissue lysate
        Lane 2 : Human thyroid tissue lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 305 kDa
        Observed band size: 330 kDa why is the actual band size different from the predicted?


        Exposure time: 37 seconds


        Blocking and dilution buffer: 5% NFDM/TBST.

        Thyroglobulin is a glycoprotein synthesised exclusively by the thyroid follicular cells. The observed molecular weight is consistent with the reported MW (PMID: 14657389).

        Negative control: Human kidney.

      • Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
        Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab243094? Please let us know so that we can cite the reference in this datasheet.

      ab243094 has not yet been referenced specifically in any publications.

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