Recombinant Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Thyroglobulin antibody [CPT-R35.3-41]
See all Thyroglobulin primary antibodies -
Description
Rabbit monoclonal [CPT-R35.3-41] to Thyroglobulin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Thyroglobulin aa 1550-1650. The exact sequence is proprietary.
Database link: P01266 -
Positive control
- WB: Human thyroid tissue lysate. ELISA: Immunizing peptide.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CPT-R35.3-41 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab243094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 330 kDa (predicted molecular weight: 305 kDa). | |
| ELISA | Use a concentration of 0.015 - 1 µg/ml. |
Target
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Function
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Tissue specificity
Thyroid gland specific. -
Involvement in disease
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
Post-translational
modificationsSulfated tyrosines are desulfated during iodination. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
- Unigene: 654591 Human
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Alternative names
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
Images
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![ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)](https://www.abcam.com/ps/products/243/ab243094/Images/ab243094-2-243094ELISA1.jpg)
ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)50ng CPTAC-39d (1000ng/mL, 50 μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243094 (50μL) were incubated with CPTAC-39d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
Secondary antibody was used at 1/2500 dilution.
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![Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)](https://www.abcam.com/ps/products/243/ab243094/Images/ab243094-1-243094WB1.jpg)
Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094)All lanes : Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Human thyroid tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 305 kDa
Observed band size: 330 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Thyroglobulin is a glycoprotein synthesised exclusively by the thyroid follicular cells. The observed molecular weight is consistent with the reported MW (PMID: 14657389).
Negative control: Human kidney.
Datasheets and documents
References
ab243094 has not yet been referenced specifically in any publications.
