Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3614] to Thyroglobulin
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Product nameAnti-Thyroglobulin antibody [EPR3614]
See all Thyroglobulin primary antibodies
DescriptionRabbit monoclonal [EPR3614] to Thyroglobulin
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Thyroglobulin aa 350-450. The exact sequence is proprietary.
- WB: Human thyroid lysate IHC-P: Human papillary thyroid carcinoma
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/20000. Predicted molecular weight: 305 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPrecursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Tissue specificityThyroid gland specific.
Involvement in diseaseDefects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains.
modificationsSulfated tyrosines are desulfated during iodination.
- Information by UniProt
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
Anti-Thyroglobulin antibody [EPR3614] (ab92467) at 1/10000 dilution + Human thyroid lysate at 10 µg
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 305 kDa
ab92467, at a 1/2500 dilution, staining Thyroglobulin in paraffin embedded Human papillary thyroid carcinoma tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab92467 has been referenced in 2 publications.
- Fernando R et al. Expression of thyrotropin receptor, thyroglobulin, sodium-iodide symporter, and thyroperoxidase by fibrocytes depends on AIRE. J Clin Endocrinol Metab 99:E1236-44 (2014). PubMed: 24708100
- Fernando R et al. Human fibrocytes coexpress thyroglobulin and thyrotropin receptor. Proc Natl Acad Sci U S A 109:7427-32 (2012). ICC/IF ; Human . PubMed: 22517745