Recombinant Anti-Thyroglobulin antibody [EPR3614(2)] (ab151539)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3614(2)] to Thyroglobulin
- Suitable for: mIHC, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Thyroglobulin antibody [EPR3614(2)]
See all Thyroglobulin primary antibodies -
Description
Rabbit monoclonal [EPR3614(2)] to Thyroglobulin -
Host species
Rabbit -
Tested applications
Suitable for: mIHC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Thyroglobulin. The exact sequence is proprietary.
-
Positive control
- Human thyroid lysate; Human thyroid gland follicular carcinoma tissue. mIHC: Human thyroid gland tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3614(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
mIHC |
1/5000.
|
|
WB |
1/100000 - 1/500000. Predicted molecular weight: 305 kDa.
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
mIHC
1/5000. |
WB
1/100000 - 1/500000. Predicted molecular weight: 305 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Tissue specificity
Thyroid gland specific. -
Involvement in disease
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
Post-translational
modificationsSulfated tyrosines are desulfated during iodination. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
- Unigene: 654591 Human
-
Alternative names
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
Images
-
Fluorescence multiplex immunohistochemical analysis of Human thyroid gland tissue (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Thyroglobulin (ab151539, magenta; Opal™690), anti-Thyroid Peroxidase/TPO (ab109383, green; Opal™520) and anti-PAX8 (ab227707, red; Opal™570) on human thyroid gland. Panel B: anti-Thyroid Peroxidase/TPO stained on cytoplasm of follicular epithelial cells. Panel C: anti-PAX8 stained on nucleus of follicular epithelial cells. Panel D: anti-Thyroglobulin stained on colloid. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of ab151539 at 1/5000 dilution (0.167 μg/ml), ab109383 at 1/1500 dilution (0.072 μg/ml) , and ab227707 at 1/200 dilution (5.22 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. DAPI (blue) was used as a nuclear counter stain.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
Immunohistochemical analysis of paraffin-embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with ab151539 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Anti-Thyroglobulin antibody [EPR3614(2)] (ab151539) at 1/100000 dilution + Human thyroid tissue lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 305 kDa -
Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151539 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded normal Human prostate tissue using ab151539 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human Thyroid gland tissue using ab151539 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab151539 has been referenced in 2 publications.
- Pan J et al. Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution. Front Cell Dev Biol 9:758339 (2021). PubMed: 34805166
- Ishido Y et al. Thyroglobulin suppresses thyroid-specific gene expression in cultures of normal but not neoplastic human thyroid follicular cells. J Clin Endocrinol Metab 99:E694-702 (2014). PubMed: 24433000