Overview

  • Product name
    Anti-Thyroglobulin antibody [EPR9730]
    See all Thyroglobulin primary antibodies
  • Description
    Rabbit monoclonal [EPR9730] to Thyroglobulin
  • Host species
    Rabbit
  • Specificity
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Thyroglobulin aa 1000-1100. The exact sequence is proprietary.
    Database link: P01266

  • Positive control
    • Mouse thyroid, rat thyroid, and Human thyroid lysates, Human thyroid gland follicular carcinoma tissue and Human thyroid gland papillary carcinoma tissue, TT cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR9730
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1:100.

WB 1/10000 - 1/50000. Detects a band of approximately 294-300 kDa (predicted molecular weight: 305 kDa).
IHC-P 1/250 - 1/500.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF 1/50 - 1/100.

Target

  • Function
    Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
  • Tissue specificity
    Thyroid gland specific.
  • Involvement in disease
    Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
    Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
  • Sequence similarities
    Belongs to the type-B carboxylesterase/lipase family.
    Contains 11 thyroglobulin type-1 domains.
  • Post-translational
    modifications
    Sulfated tyrosines are desulfated during iodination.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AITD 3 antibody
    • AITD3 antibody
    • hTG antibody
    • TDH3 antibody
    • Tg antibody
    • TGN antibody
    • THYG_HUMAN antibody
    • Thyroglobulin antibody
    see all

Images

  • Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified) + Rat thyroid lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified)

    Lane 1 : Mouse thyroid lysates
    Lane 2 : ICR thyroid lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (purified) + Human thyroid lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.

    See Abreview

  • All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (unpurified)

    Lane 1 : Mouse thyroid lysate
    Lane 2 : Rat thyroid lysate
    Lane 3 : Human thyroid lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 305 kDa
    Observed band size: 294-300 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

  • Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

  • Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

References

This product has been referenced in:
  • Yang J  et al. Generation and characterization of a hypothyroidism rat model with truncated thyroid stimulating hormone receptor. Sci Rep 8:4004 (2018). Read more (PubMed: 29507327) »
  • Shimamura M  et al. Mouse models of sporadic thyroid cancer derived from BRAFV600E alone or in combination with PTEN haploinsufficiency under physiologic TSH levels. PLoS One 13:e0201365 (2018). Read more (PubMed: 30086162) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
Formaldehyde
Sample
Mouse Cell (thyroid)
Specification
thyroid
Gating Strategy
epithelial cells
Permeabilization
Yes - BD permeabilization buffer

Mr. Sanjay Gawade

Verified customer

Submitted Mar 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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