Recombinant Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (ab214872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR9730] to Thyroglobulin
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730]
See all Thyroglobulin primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR9730] to Thyroglobulin -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Thyroglobulin aa 1000-1100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01266 -
Positive control
- ICC/IF: TT cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9730 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Thyroglobulin antibody [EPR9730] (ab156008)
- Anti-Thyroglobulin antibody [EPR9730(IHC)] (ab168344)
- APC Anti-Thyroglobulin antibody [EPR9730] (ab221284)
- Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
- Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
This product gave a positive signal in TT cells fixed with 100% methanol (5 min) |
Notes |
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ICC/IF
1/50. This product gave a positive signal in TT cells fixed with 100% methanol (5 min) |
Target
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Function
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Tissue specificity
Thyroid gland specific. -
Involvement in disease
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
Post-translational
modificationsSulfated tyrosines are desulfated during iodination. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7038 Human
- Entrez Gene: 21819 Mouse
- Entrez Gene: 24826 Rat
- Omim: 188450 Human
- SwissProt: P01266 Human
- SwissProt: O08710 Mouse
- SwissProt: P06882 Rat
- Unigene: 654591 Human
see all -
Alternative names
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
Images
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ab214872 staining Thyroglobulin in TT cells. The cells were fixed with 100% methanol (5 min), and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions.
The cells were then incubated overnight at +4°C with ab214872 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab214872 has not yet been referenced specifically in any publications.