Recombinant
RabMAb

Recombinant Anti-Thyroglobulin antibody [EPR9730] (Allophycocyanin) (ab221284)

Overview

  • Product name

    Anti-Thyroglobulin antibody [EPR9730] (Allophycocyanin)
    See all Thyroglobulin primary antibodies
  • Description

    Rabbit monoclonal [EPR9730] to Thyroglobulin (Allophycocyanin)
  • Host species

    Rabbit
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Thyroglobulin aa 1000-1100. The exact sequence is proprietary.
    Database link: P01266

  • Positive control

    • Flow Cyt: TT cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9730
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
  • Tissue specificity

    Thyroid gland specific.
  • Involvement in disease

    Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
    Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
    Contains 11 thyroglobulin type-1 domains.
  • Post-translational
    modifications

    Sulfated tyrosines are desulfated during iodination.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AITD 3 antibody
    • AITD3 antibody
    • hTG antibody
    • TDH3 antibody
    • Tg antibody
    • TGN antibody
    • THYG_HUMAN antibody
    • Thyroglobulin antibody
    see all

Images

  • Overlay histogram showing TT cells stained with ab221284 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221284, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 17 mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

References

ab221284 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221284.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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