Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
Product nameAnti-Thyroglobulin antibody [EPR9730(IHC)]
See all Thyroglobulin primary antibodies
DescriptionRabbit monoclonal [EPR9730(IHC)] to Thyroglobulin
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Unsuitable for: IP or WB
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant full length protein corresponding to Human Thyroglobulin aa 1-2800.
Database link: P01266
- Human thyroid gland follicular and papillary carcinoma tissues; TT cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Anti-Thyroglobulin antibody [EPR9730] (ab156008)
- Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (ab214872)
- Alexa Fluor® 647 Anti-Thyroglobulin antibody [EPR9730] (ab215938)
- Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
- Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
Our Abpromise guarantee covers the use of ab168344 in the following tested applications.
|ICC/IF||1/250 - 1/500.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPrecursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
Tissue specificityThyroid gland specific.
Involvement in diseaseDefects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains.
modificationsSulfated tyrosines are desulfated during iodination.
- Information by UniProt
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
ab168344 showing +ve staining in Human thyroid gland tissue.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland papillary carcinoma tissue labelingThyroglobulin with ab168344 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with ab168344 at 1/250 dilution.
ab168344 showing -ve staining in Human skeletal muscle tissue.
ab168344 showing -ve staining in Human normal kidney tissue.
ab168344 showing -ve staining in Human normal tonsil tissue.
ab168344 showing -ve staining in Human prostate hyperplasia tissue.
Immunofluorescent analysis of TT cells labeling Thyroglobulin with ab168344 at 1/250 dilution.
ab168344 has not yet been referenced specifically in any publications.