Recombinant Anti-Thyroglobulin antibody [EPR9730(IHC)] (ab168344)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Thyroglobulin antibody [EPR9730(IHC)]
See all Thyroglobulin primary antibodies -
Description
Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Thyroglobulin aa 1-2800.
Database link: P01266 -
Positive control
- Human thyroid gland follicular and papillary carcinoma tissues; TT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9730(IHC) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/250 - 1/500.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/250 - 1/500. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Tissue specificity
Thyroid gland specific. -
Involvement in disease
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
Post-translational
modificationsSulfated tyrosines are desulfated during iodination. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
- Unigene: 654591 Human
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Alternative names
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
Images
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ab168344 showing +ve staining in Human thyroid gland tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human thyroid gland papillary carcinoma tissue labelingThyroglobulin with ab168344 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with ab168344 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab168344 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab168344 showing -ve staining in Human normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab168344 showing -ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab168344 showing -ve staining in Human prostate hyperplasia tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of TT cells labeling Thyroglobulin with ab168344 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168344 has not yet been referenced specifically in any publications.