Recombinant
RabMAb

Recombinant Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

Submit a review Submit a question

Overview

  • Product name

    Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free
    See all Thyroglobulin primary antibodies

  • Description

    Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human Thyroglobulin aa 1-2800.
    Database link: P01266

  • General notes

    Ab249444 is the carrier-free version of ab168344. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249444 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9730(IHC)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab249444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP or WB.

    • Target

    • Function

      Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

    • Tissue specificity

      Thyroid gland specific.

    • Involvement in disease

      Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
      Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.

    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.
      Contains 11 thyroglobulin type-1 domains.

    • Post-translational
      modifications

      Sulfated tyrosines are desulfated during iodination.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • AITD 3 antibody
        • AITD3 antibody
        • hTG antibody
        • TDH3 antibody
        • Tg antibody
        • TGN antibody
        • THYG_HUMAN antibody
        • Thyroglobulin antibody
        see all

      References

      ab249444 has not yet been referenced specifically in any publications.

      Publishing research using ab249444? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab249444.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com