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RecombinantRabMAb

Recombinant Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

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  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Immunocytochemistry - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
  • Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin - BSA and Azide free
  • Suitable for: IHC-P, ICC
  • Reacts with: Human

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Recombinant Human Thyroglobulin protein (ab152743)
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Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (ab269822)

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Overview

  • Product name

    Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free
    See all Thyroglobulin primary antibodies
  • Description

    Rabbit monoclonal [EPR9730(IHC)] to Thyroglobulin - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Thyroglobulin aa 1-2800.
    Database link: P01266

  • General notes

    Ab249444 is the carrier-free version of ab168344. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249444 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9730(IHC)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Tumor biomarkers
    • Globulins

Associated products

  • Alternative Versions

    • Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    • Anti-Thyroglobulin antibody [EPR9730(IHC)] (ab168344)
    • Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (ab214872)
    • Alexa Fluor® 647 Anti-Thyroglobulin antibody [EPR9730] (ab215938)
    • Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Thyroglobulin protein (ab152743)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP or WB.
  • Target

    • Function

      Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
    • Tissue specificity

      Thyroid gland specific.
    • Involvement in disease

      Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
      Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.
    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.
      Contains 11 thyroglobulin type-1 domains.
    • Post-translational
      modifications

      Sulfated tyrosines are desulfated during iodination.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01266 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7038 Human
      • Omim: 188450 Human
      • SwissProt: P01266 Human
      • Unigene: 654591 Human
      • Alternative names

        • AITD 3 antibody
        • AITD3 antibody
        • hTG antibody
        • TDH3 antibody
        • Tg antibody
        • TGN antibody
        • THYG_HUMAN antibody
        • Thyroglobulin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

        This data was developed using ab168344, the same antibody clone in a different buffer formulation.

        ab168344 showing +ve staining in Human thyroid gland tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

        This data was developed using ab168344, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Human thyroid gland papillary carcinoma tissue labelingThyroglobulin with ab168344 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

        This data was developed using ab168344, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with ab168344 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

        This data was developed using ab168344, the same antibody clone in a different buffer formulation.

        ab168344 showing -ve staining in Human skeletal muscle tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        This data was developed using ab168344, the same antibody clone in a different buffer formulation.ab168344 showing -ve staining in Human normal kidney tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        This data was developed using ab168344, the same antibody clone in a different buffer formulation.ab168344 showing -ve staining in Human normal tonsil tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        This data was developed using ab168344, the same antibody clone in a different buffer formulation.ab168344 showing -ve staining in Human prostate hyperplasia tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunocytochemistry - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Immunocytochemistry - Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        This data was developed using ab168344, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of TT cells labeling Thyroglobulin with ab168344 at 1/250 dilution.
      • Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
        Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab249444? Please let us know so that we can cite the reference in this datasheet.

      ab249444 has not yet been referenced specifically in any publications.

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