Product nameAnti-Thyroid Hormone Receptor alpha 1+2 antibody
See all Thyroid Hormone Receptor alpha 1+2 primary antibodies
DescriptionRabbit polyclonal to Thyroid Hormone Receptor alpha 1+2
Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig, Zebrafish
- Jurkat cell lysate and human skin tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
Our Abpromise guarantee covers the use of ab42565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 58, 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:62500.
FunctionIsoform Alpha-1: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Isoform Alpha-2: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action.
Involvement in diseaseHypothyroidism, congenital, non-goitrous, 6
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- c-erbA-1 antibody
- C-erbA-alpha antibody
- EAR-7 antibody
Anti-Thyroid Hormone Receptor alpha 1+2 antibody (ab42565) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 58, 47 kDa
Observed band size: 47,58 kDa why is the actual band size different from the predicted?
Gel concentration: 12% Predicted band size: isoform 1: 47kDa; isoform 2: 55kDa
ab42565 at 4ug/ml concentration, staining human THRA in squamous epithelial cells of human skin (indicated by arrows) by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
ab42565 has not yet been referenced specifically in any publications.