Product nameAnti-Thyroid Hormone Receptor alpha antibody
See all Thyroid Hormone Receptor alpha primary antibodies
DescriptionRabbit polyclonal to Thyroid Hormone Receptor alpha
Specificityab53729 detects endogenous levels of total Thyroid Hormone Receptor alpha 1 protein.
Tested applicationsSuitable for: WB, IHC-P, ELISA, ChIP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from human Thyroid Hormone Receptor alpha.
- IHC-P: Human colon carcinoma tissue; WB: SKOV3 cell extracts. ChIP: C2C12
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
Our Abpromise guarantee covers the use of ab53729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
FunctionNuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Involvement in diseaseHypothyroidism, congenital, non-goitrous, 6
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
- AR7 antibody
- c-erbA-1 antibody
- C-erbA-alpha antibody
Chromatin was prepared from C2C12 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab53729 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
All lanes : Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/500 dilution
Lane 1 : SKOV3 cell extract
Lane 2 : SKOV3 cell extract with immunising peptide
Predicted band size: 55 kDa
Observed band size: 55 kDa
ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Liang X et al. Thyroid function alterations attributed to high iodide supplementation in maternal rats and their offspring. J Trace Elem Med Biol 47:89-97 (2018). Read more (PubMed: 29544812) »
- Howie RN et al. Effects of In Utero Thyroxine Exposure on Murine Cranial Suture Growth. PLoS One 11:e0167805 (2016). IHC-P ; Mouse . Read more (PubMed: 27959899) »