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Rabbit polyclonal to Thyroid Hormone Receptor alpha
ab53729 detects endogenous levels of total Thyroid Hormone
Receptor alpha 1 protein.
Mouse, Rat, Human
Synthetic peptide derived from human Thyroid Hormone Receptor alpha.
Human colon carcinoma tissue; SKOV3 cell extracts.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS Without Mg+2 and Ca+2
Concentration information loading...
Immunogen affinity purified
ab53729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Use at an assay dependent concentration.
Use a concentration of 1 - 5 µg/ml.
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Involvement in disease
Hypothyroidism, congenital, non-goitrous, 6
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
All lanes : Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/500 dilution Lane 1 : SKOV3 cell extract Lane 2 : SKOV3 cell extract with immunising peptide Predicted band size: 55 kDa Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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