Overview

  • Product name
    Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade
    See all Thyroid Hormone Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Thyroid Hormone Receptor beta - ChIP Grade
  • Host species
    Rabbit
  • Specificity
    This antibody does not detect TR alpha-1 or TRv alpha-2.
  • Tested applications
    Suitable for: ICC/IF, ELISA, ChIP, IHC-P, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81. With an N-terminal added cysteine.
    Sequence:

    IFHLDHDDVNDQSVSSAQTF

Properties

Applications

Our Abpromise guarantee covers the use of ab5622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
ELISA Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
ICC 1/50 - 1/500.
WB 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).

representing recombinant human TRv beta-1 expressed in E. coli

EMSA Use at an assay dependent concentration.

Target

  • Function
    High affinity receptor for triiodothyronine.
  • Involvement in disease
    Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
    Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
    • C ERBA 2 antibody
    • C ERBA BETA antibody
    • c-erbA-2 antibody
    • c-erbA-beta antibody
    • ERBA 2 antibody
    • ERBA BETA antibody
    • ERBA2 antibody
    • Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian antibody
    • generalized resistance to thyroid hormone antibody
    • GRTH antibody
    • MGC126109 antibody
    • MGC126110 antibody
    • NR1A2 antibody
    • Nuclear receptor subfamily 1 group A member 2 antibody
    • Oncogene ERBA2 antibody
    • PRTH antibody
    • THB_HUMAN antibody
    • THR1 antibody
    • THRB 1 antibody
    • THRB 2 antibody
    • thrB antibody
    • THRB1 antibody
    • THRB2 antibody
    • Thyroid hormone nuclear receptor beta variant 1 antibody
    • Thyroid hormone receptor beta 1 antibody
    • Thyroid hormone receptor beta 2 antibody
    • Thyroid hormone receptor beta antibody
    • Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) antibody
    see all

Images

  • ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab5622 staining Thyroid Hormone Receptor in Human colon tissue sections (right) compared to negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 1 hour at 37°C. A HRP-conjugated secondary antibody was used for detection.

  • ab5622 staining Thyroid Hormone Receptor beta in A431 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

  • ab5622 staining Thyroid Hormone Receptor beta in Hela cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at room temperature. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

  • ab5622 staining Thyroid Hormone Receptor beta in L6 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

References

This product has been referenced in:
  • Mahaman YAR  et al. Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments. J Alzheimers Dis 63:1141-1159 (2018). Read more (PubMed: 29710724) »
  • Yakoub AM & Sadek M Development and Characterization of Human Cerebral Organoids: An Optimized Protocol. Cell Transplant 27:393-406 (2018). Read more (PubMed: 29749250) »
See all 7 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your email.
Yes, I will arrange for the shipment via the distributor through which the antibody ab5622 was originally purchased.

On the original order I saw that 2 vials had been ordered. Could you please let me know whether you had ordered 2 or just 1 vial?
In case you had ordered2 vials, would you like me to send you 2 vials of ab53170?

Please let me know and I will arrange for everything.

I look forward to hear back from you.

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Answer

Thank you for the additional information.

I found an order from xxx. Can you please confirm that you ordered the antibody via xxx?

Our guarantee is valid only for 6 months after the purchase (https://www.abcam.com/Abpromise). But I'd be happy to make a one-time exception and can offer you either a free of charge replacement with another antibody (e.g. Thyroid-Hormone-Receptor-beta-antibody-ab106813.html, Thyroid-Hormone-Receptor-beta-antibody-ab53170.htmlor Thyroid-Hormone-Receptor-beta-antibody-ab33544.html)or a credit.

Please let me know which option would be best for you.

I look forward to hear back.

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Thank you for contacting us.

I am sorry to hear that the antibody is not working very well.

Could you please help me answer a few more questions:
1) What samples are you using and which species are they from?
2) Have you tried a no primary control to see if the secondary antibody contributes to or causes the high background?
3) When did you order the antibody and what is your order number?

I look forward to hear back from you and assist you further.

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Answer

Thank you for contacting us. Your credit note IDs are XXX. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

I can confirm that the sentence regarding TRv alpha-2 was a datasheet error. Thank you for bringing this to our attention. The information should read, "this antibody detects a 55 kDa protein representing recombinant human TRv Beta-1 expressed in E. coli." and it has been corrected immediately.

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Answer

Thank you for your enquiry. Unfortunately, we don't have the corresponding peptides for these antibodies. If you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). Should you require any further information, please get in touch with us and we will gladly assist you.

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