Product nameAnti-Thyroid Hormone Receptor beta antibody - N-terminal
See all Thyroid Hormone Receptor beta primary antibodies
DescriptionRabbit polyclonal to Thyroid Hormone Receptor beta - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep
Recombinant fragment corresponding to Human Thyroid Hormone Receptor beta 1 aa 1-250 (N terminal).
MTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSERRSTLK NEQSSPHLIQTTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSY LDKDELCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYEGK CVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRR REELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDI
Database link: P10828
- WB: HepG2 and U-87MG cell lysates. Mouse liver and eye tissue lysates. Rat liver and eye tissue lysates. ICC/ IF: U-2 OS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 53 kDa.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/200.|
FunctionHigh affinity receptor for triiodothyronine.
Involvement in diseaseDefects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
- Information by UniProt
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Immunofluorescence staining of U-2 OS cells stained for Thyroid Hormone Receptor beta with ab180612 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-Thyroid Hormone Receptor beta antibody - N-terminal (ab180612) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U-87MG cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse eye tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 6 : Rat eye tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat AntiRabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 seconds
Blocking buffer: 3% nonfat dry milk in TBST