Anti-Thyroid Peroxidase antibody [MoAb47] (ab12500)


  • Product name
    Anti-Thyroid Peroxidase antibody [MoAb47]
    See all Thyroid Peroxidase primary antibodies
  • Description
    Mouse monoclonal [MoAb47] to Thyroid Peroxidase
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Thyroid Peroxidase purified from thyroid microsomes by immunoaffinity chromatography

  • Positive control
    • Thyroid



Our Abpromise guarantee covers the use of ab12500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/10 - 1/25 in an ABC method. Perform heat mediated antigen retrieval with 1mM EDTA buffer, pH 9.0 before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
    • Pathway
      Hormone biosynthesis; thyroid hormone biosynthesis.
    • Involvement in disease
      Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
      Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
    • Sequence similarities
      Belongs to the peroxidase family. XPO subfamily.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • Post-translational
      Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
      Cleaved in its N-terminal part.
    • Cellular localization
      Membrane and Cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • MSA antibody
      • PERT_HUMAN antibody
      • TDH2A antibody
      • Thyroid microsomal antigen antibody
      • Thyroid peroxidase antibody
      • Thyroperoxidase antibody
      • TPO antibody
      • TPX antibody
      see all


    This product has been referenced in:
    • Cairns DM  et al. Muscle cells enhance resistance to pro-inflammatory cytokine-induced cartilage destruction. Biochem Biophys Res Commun 392:22-8 (2010). Read more (PubMed: 20043873) »
    • Cairns DM  et al. The role of muscle cells in regulating cartilage matrix production. J Orthop Res 28:529-36 (2010). Read more (PubMed: 19813241) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry.

    I can confirm that ab12500 Anti-Thyroid Peroxidase antibody [MoAb47]antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernata...

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    Thank you for contacting us.

    According to the datasheet, this antibody is a non-purified cell culture supernatant product. That means, along with whole serum andascitis products, the antibody concentration cannot be determined as there are t...

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    Thank you for providing some further information. I understand your customer has used up most of the product during testing and optimization. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vi...

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    Thank you for getting back to me and for providing some further details. I understand that whilst ab818 failed to work In Western blot and in Dot blot; the other antibody ab12500 seemed to be fine. It is important to mention that these antibodies detec...

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    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has...

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