Overview

  • Product name

    Anti-Thyroid Peroxidase ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 20 3.8%
    Pos. Serum 20 4.8%
    Neg. Serum 20 6%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 113.3U/ml 7.1%
    Pos. Serum 12 176U/ml 5.7%
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Indirect
  • Sensitivity

    < 0.5 U/ml
  • Range

    5 U/ml - 320 U/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Thyroid Peroxidase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroid peroxidase in Human serum or plasma (citrate).


    A 96-well plate has been precoated with recombinant thyroid peroxidase to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroid peroxidase-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroid peroxidase sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover Foil 1 unit
    IgG Sample Diluent 1 x 100ml
    Stop Solution red cap 1 x 15ml
    Strip holder 1 unit
    Thyroid peroxidase Anti-IgG HRP Conjugate 1 x 20ml
    Thyroid peroxidase Coated Microplate (12 x 8 wells) 1 unit
    Thyroid peroxidase Negative Control 1 x 2ml
    Thyroid peroxidase Positive Control 1 x 2ml
    Thyroid peroxidase Standard A - 0 U/mL 1 x 2ml
    Thyroid peroxidase Standard B - 5 U/mL 1 x 2ml
    Thyroid peroxidase Standard C - 10 U/mL 1 x 2ml
    Thyroid peroxidase Standard D - 20 U/mL 1 x 2ml
    Thyroid peroxidase Standard E - 80 U/mL 1 x 2ml
    Thyroid peroxidase Standard F - 320 U/mL 1 x 2ml
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178632

Protocols

References

This product has been referenced in:

  • Landriscina M  et al. RAS/BRAF mutational status in familial non-medullary thyroid carcinomas: A retrospective study. Oncol Lett 10:1875-1881 (2015). Read more (PubMed: 26622768) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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