Key features and details
- Rabbit polyclonal to Thyroid Peroxidase/TPO
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Thyroid Peroxidase/TPO antibody
See all Thyroid Peroxidase/TPO primary antibodies
DescriptionRabbit polyclonal to Thyroid Peroxidase/TPO
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human thyroid tissue, Rat thyroid lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
|WB||1/100 - 1/1000. Predicted molecular weight: 101 kDa.|
FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
PathwayHormone biosynthesis; thyroid hormone biosynthesis.
Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part.
Cellular localizationMembrane and Cell surface.
- Information by UniProt
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
Anti-Thyroid Peroxidase/TPO antibody (ab203057) at 1/200 dilution + Rat thyroid lysate
Predicted band size: 101 kDa
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human thyroid tissue labeling Thyroid Peroxidase/TPO with ab203057 at 1/200 dilution. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.
ab203057 has been referenced in 1 publication.
- Yang J et al. Generation and characterization of a hypothyroidism rat model with truncated thyroid stimulating hormone receptor. Sci Rep 8:4004 (2018). PubMed: 29507327