Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody
    See all Thyroid Peroxidase/TPO primary antibodies
  • Description

    Rabbit polyclonal to Thyroid Peroxidase/TPO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse Thyroid Peroxidase/TPO aa 375-425 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    TPCFLA GDGRASEVPA LAAVHTLWLR EHNRLASAFK AINKHWSANT AYQEA


    Database link: P35419

  • Positive control

    • Human thyroid tissue, Rat thyroid lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab203057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 101 kDa.

Target

  • Function

    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • Pathway

    Hormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in disease

    Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications

    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localization

    Membrane and Cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Images

  • Anti-Thyroid Peroxidase/TPO antibody (ab203057) at 1/200 dilution + Rat thyroid lysate

    Predicted band size: 101 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human thyroid tissue labeling Thyroid Peroxidase/TPO with ab203057 at 1/200 dilution. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

References

This product has been referenced in:

  • Yang J  et al. Generation and characterization of a hypothyroidism rat model with truncated thyroid stimulating hormone receptor. Sci Rep 8:4004 (2018). Read more (PubMed: 29507327) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Thyroid gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat Buffer
Permeabilization
No
Specification
Thyroid gland
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Miguel Campos

Verified customer

Submitted Aug 28 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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