Recombinant Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Thyroid Peroxidase/TPO antibody [EPR5379]
See all Thyroid Peroxidase/TPO primary antibodies -
Description
Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human thyroid lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5379 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/10000 - 1/20000. Predicted molecular weight: 103 kDa.
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IP |
1/40.
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Notes |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/10000 - 1/20000. Predicted molecular weight: 103 kDa. |
IP
1/40. |
Target
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Function
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). -
Pathway
Hormone biosynthesis; thyroid hormone biosynthesis. -
Involvement in disease
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsGlycosylated.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part. -
Cellular localization
Membrane and Cell surface. - Information by UniProt
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Database links
- Entrez Gene: 7173 Human
- Omim: 606765 Human
- SwissProt: P07202 Human
- Unigene: 467554 Human
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Alternative names
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
see all
Images
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Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/20000 dilution (purified) + Human thyroid tissue lysate at 10 µg
Secondary
HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase/TPO in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 103 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab133322 has been referenced in 4 publications.
- Liu Y et al. Pyruvate carboxylase promotes malignant transformation of papillary thyroid carcinoma and reduces iodine uptake. Cell Death Discov 8:423 (2022). PubMed: 36266265
- Cui X et al. Circulating Exosomes Activate Dendritic Cells and Induce Unbalanced CD4+ T Cell Differentiation in Hashimoto Thyroiditis. J Clin Endocrinol Metab 104:4607-4618 (2019). PubMed: 31199456
- Guria S et al. Functional analysis of thyroid peroxidase gene mutations detected in patients with thyroid dyshormonogenesis. Int J Endocrinol 2014:390121 (2014). PubMed: 24829575
- Ishido Y et al. Thyroglobulin suppresses thyroid-specific gene expression in cultures of normal but not neoplastic human thyroid follicular cells. J Clin Endocrinol Metab 99:E694-702 (2014). PubMed: 24433000