Product nameAnti-Thyroid Peroxidase/TPO antibody [EPR5379]
See all Thyroid Peroxidase/TPO primary antibodies
DescriptionRabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human Thyroid Peroxidase/TPO aa 1-100 (extracellular). The exact sequence is proprietary.
- Human thyroid lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/10000 - 1/20000. Predicted molecular weight: 103 kDa.|
FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
PathwayHormone biosynthesis; thyroid hormone biosynthesis.
Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part.
Cellular localizationMembrane and Cell surface.
- Information by UniProt
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/20000 dilution (purified) + Human thyroid tissue lysate at 10 µg
HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase/TPO in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 103 kDa