Recombinant
RabMAb

Recombinant Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322)

Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody [EPR5379]
    See all Thyroid Peroxidase/TPO primary antibodies
  • Description

    Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thyroid Peroxidase/TPO aa 1-100 (extracellular). The exact sequence is proprietary.

  • Positive control

    • Human thyroid lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/10000 - 1/20000. Predicted molecular weight: 103 kDa.
IP 1/40.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
    • Pathway

      Hormone biosynthesis; thyroid hormone biosynthesis.
    • Involvement in disease

      Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
      Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • Post-translational
      modifications

      Glycosylated.
      Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
      Cleaved in its N-terminal part.
    • Cellular localization

      Membrane and Cell surface.
    • Information by UniProt
    • Database links

    • Alternative names

      • MSA antibody
      • PERT_HUMAN antibody
      • TDH2A antibody
      • Thyroid microsomal antigen antibody
      • Thyroid peroxidase antibody
      • Thyroperoxidase antibody
      • TPO antibody
      • TPX antibody
      see all

    Images

    • Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/20000 dilution (purified) + Human thyroid tissue lysate at 10 µg

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase/TPO in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 103 kDa

    References

    ab133322 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for contacting us. Your credit note ID is xxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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    Answer

    Thank you for contacting Abcam.

    I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol with details such as sample preparation steps, blocking procedures, antibody incubation steps, washes?

    These details will not only help me to better understand your difficulties and be able to offer suggestions but will also be used for internal testing of the products if deemed necessary. If we are unable to solve the problems you are experiencing we will replace or refund the antibody according to our Abpromise guarantee if you have purchased this product within the last six months.

    I will also try and contact the lab regarding the immunogen for this product and will inform you when I have that.

    Please let me know if you have any questions.

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