RecombinantRabMAb

Recombinant Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
  • Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
  • Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free
    See all Thyroid Peroxidase/TPO primary antibodies

  • Description

    Rabbit monoclonal [EPR5379] to Thyroid Peroxidase/TPO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248474 is the carrier-free version of ab133322 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248474 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

    • Pathway

      Hormone biosynthesis; thyroid hormone biosynthesis.

    • Involvement in disease

      Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
      Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.

    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.

    • Post-translational
      modifications

      Glycosylated.
      Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
      Cleaved in its N-terminal part.

    • Cellular localization

      Membrane and Cell surface.
    • Information by UniProt

    • Database links

      • Alternative names

        • MSA antibody
        • PERT_HUMAN antibody
        • TDH2A antibody
        • Thyroid microsomal antigen antibody
        • Thyroid peroxidase antibody
        • Thyroperoxidase antibody
        • TPO antibody
        • TPX antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        This data was developed using ab133322, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
      • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/20000 dilution (purified) + Human thyroid tissue lysate at 10 µg

        Secondary
        HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 103 kDa
        Observed band size: 103 kDa



        This data was developed using ab133322, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        This data was developed using ab133322, the same antibody clone in a different buffer formulation.ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase/TPO in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000). Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration: 5% NFDM /TBST.
      • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Anti-Thyroid Peroxidase/TPO antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 103 kDa



        This data was developed using ab133322, the same antibody clone in a different buffer formulation.

         

      • Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)
        Anti-Thyroid Peroxidase/TPO antibody [EPR5379] - BSA and Azide free (ab248474)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248474? Please let us know so that we can cite the reference in this datasheet.

      ab248474 has not yet been referenced specifically in any publications.

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