Product nameAnti-Thyroid Peroxidase/TPO antibody [EPR5380]
See all Thyroid Peroxidase/TPO primary antibodies
DescriptionRabbit monoclonal [EPR5380] to Thyroid Peroxidase/TPO
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Thyroid Peroxidase/TPO (C terminal). The exact sequence is proprietary.
- WB: Human thyroid lysate IHC-P: Human thyroid gland tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 103 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
PathwayHormone biosynthesis; thyroid hormone biosynthesis.
Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part.
Cellular localizationMembrane and Cell surface.
- Information by UniProt
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383) at 1/1000 dilution + Human thyroid lysate at 10 µg
Predicted band size: 103 kDa
ab109383, at a 1/100 dilution, staining Thyroid Peroxidase/TPO in paraffin-embedded Human thyroid gland tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Zane M et al. Estrogen and thyroid cancer is a stem affair: A preliminary study. Biomed Pharmacother 85:399-411 (2017). ICC/IF . Read more (PubMed: 27899250) »
- Fernando R et al. Expression of thyrotropin receptor, thyroglobulin, sodium-iodide symporter, and thyroperoxidase by fibrocytes depends on AIRE. J Clin Endocrinol Metab 99:E1236-44 (2014). Read more (PubMed: 24708100) »