Recombinant
RabMAb

Recombinant Anti-Thyroid Peroxidase/TPO antibody [EPR5380] - BSA and Azide free (ab239972)

Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody [EPR5380] - BSA and Azide free
    See all Thyroid Peroxidase/TPO primary antibodies
  • Description

    Rabbit monoclonal [EPR5380] to Thyroid Peroxidase/TPO - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thyroid Peroxidase/TPO (C terminal). The exact sequence is proprietary.

  • General notes

    ab239972 is the carrier-free version of ab109383 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 103 kDa.

Target

  • Function

    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • Pathway

    Hormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in disease

    Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications

    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localization

    Membrane and Cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • MSA antibody
    • PERT_HUMAN antibody
    • TDH2A antibody
    • Thyroid microsomal antigen antibody
    • Thyroid peroxidase antibody
    • Thyroperoxidase antibody
    • TPO antibody
    • TPX antibody
    see all

Images

  • ab109383, at a 1/100 dilution, staining Thyroid Peroxidase/TPO in paraffin-embedded Human thyroid gland tissue

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109383).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab239972 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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