Key features and details
- Mouse monoclonal [TPO/1922] to Thyroid Peroxidase/TPO
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Thyroid Peroxidase/TPO antibody [TPO/1922]
See all Thyroid Peroxidase/TPO primary antibodies
DescriptionMouse monoclonal [TPO/1922] to Thyroid Peroxidase/TPO
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Thyroid Peroxidase/TPO aa 685-804. The exact sequence is proprietary.
Database link: P07202
- IHC-P: Human thyroid carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab236561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
FunctionIodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
PathwayHormone biosynthesis; thyroid hormone biosynthesis.
Involvement in diseaseNote=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part.
Cellular localizationMembrane and Cell surface.
- Information by UniProt
- MSA antibody
- PERT_HUMAN antibody
- TDH2A antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236561 has not yet been referenced specifically in any publications.