Product nameAnti-Thyroxine Binding Globulin antibody
See all Thyroxine Binding Globulin primary antibodies
DescriptionRabbit polyclonal to Thyroxine Binding Globulin
Tested applicationsSuitable for: EIA, RIA, IP, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Dog, Human
Human TBG purified from human plasma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab48646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EIA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
- Information by UniProt
- Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
- Serpin A7 antibody
- Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
ICC/IF image of ab48646 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48646, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab48646 recognizes the full length recombinant thyroxine binding globulin protein which has an expected molecular weight of 55 kDa due to glycosylation.
ab48646 has not yet been referenced specifically in any publications.