Anti-Thyroxine Binding Globulin antibody (ab48646)
Key features and details
- Rabbit polyclonal to Thyroxine Binding Globulin
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Thyroxine Binding Globulin antibody
See all Thyroxine Binding Globulin primary antibodies -
Description
Rabbit polyclonal to Thyroxine Binding Globulin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Human TBG purified from human plasma
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Positive control
- ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 6906 Human
- Omim: 314200 Human
- SwissProt: P05543 Human
- Unigene: 76838 Human
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Alternative names
- Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
- Serpin A7 antibody
- Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thyroxine Binding Globulin antibody (ab48646)ICC/IF image of ab48646 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48646, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Thyroxine Binding Globulin antibody (ab48646)Ab48646 recognizes the full length recombinant thyroxine binding globulin protein which has an expected molecular weight of 55 kDa due to glycosylation.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab48646 has not yet been referenced specifically in any publications.