Key features and details
- Sensitivity: 0.004 µg/ml
- Range: 0.044 µg/ml - 0.108 µg/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameThyroxine (Total T4) ELISA Kit
See all Thyroxine (T4) kits
Intra-assay Sample n Mean SD CV% Sera 20 <=8.16% Inter-assay Sample n Mean SD CV% Sera 10 <=8.42%
Sample typeSerum, Hep Plasma, Cit plasma
Sensitivity> 0.004 µg/ml
Range0.044 µg/ml - 0.108 µg/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of T4 in serum and plasma.
A 96-well plate has been precoated with anti-Thyroxine (T4) antibodies (solid-phase). Samples and the Thyroxine (T4)-HRP conjugate are added to the wells, where Thyroxine (T4) in the sample competes with the added Thyroxine (T4)-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine (T4) in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 50X Washing Solution 1 x 20ml Anti-Thyroxine IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover Foil 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Thyroxine HRP Conjugate 1 x 1.4ml Thyroxine Standard 0 - 0 µg/mL 1 x 1ml Thyroxine Standard 1 1 x 1ml Thyroxine Standard 2 - 0.050 µg/mL 1 x 1ml Thyroxine Standard 3 - 0.100 µg/mL 1 x 1ml Thyroxine Standard 4 - 0.150 µg/mL 1 x 1ml Thyroxine Standard 5 - 0.250 µg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml
- SwissProt: P05543 Human
ab178664 has been referenced in 2 publications.
- Gil-Cayuela C et al. Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis. PLoS One 13:e0190987 (2018). PubMed: 29320567
- Moura SL et al. Theoretical and experimental study for the biomimetic recognition of levothyroxine hormone on magnetic molecularly imprinted polymer. Biosens Bioelectron 107:203-210 (2018). PubMed: 29471281