Anti-TIA1 antibody (ab205063)
Key features and details
- Rabbit polyclonal to TIA1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIA1 antibody
See all TIA1 primary antibodies -
Description
Rabbit polyclonal to TIA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TIA1 aa 8-193. from Isoform 3: BC015944
Sequence:TLY VGNLSRDVTE ALILQLFSQI GPCKNCKMIM DTAGNDPYCF VEFHEHRHAA AALAAMNGRK IMGKEVKVNW ATTPSSQKKD TSSSTVVSTQ RSQDHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN WATRKPPAPK STY
Database link: P31483-3 -
Positive control
- K562 cell lysate. Human brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. | |
| WB | 1/200 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43, 42, 24 kDa). |
Target
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Function
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. - Information by UniProt
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Database links
- Entrez Gene: 7072 Human
- Omim: 603518 Human
- SwissProt: P31483 Human
- Unigene: 413123 Human
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Alternative names
- Cytotoxic granule associated RNA binding protein 1 antibody
- Cytotoxic granule associated RNA binding protein antibody
- mTIA-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody (ab205063)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling TIA1 with ab205063 at 1/100 dilution.
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Western blot - Anti-TIA1 antibody (ab205063)Anti-TIA1 antibody (ab205063) at 1/500 dilution + K562 cell lysate
Predicted band size: 43, 42, 24 kDa
Protocols
References (0)
ab205063 has not yet been referenced specifically in any publications.
