Anti-TIA1 antibody (ab61700)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TIA1 antibody
See all TIA1 primary antibodies -
Description
Goat polyclonal to TIA1 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-PKSTYESNTKQ
, corresponding to internal sequence amino acids 189-199 of Human TIA1 -
Positive control
- Jurkat whole cell lysate (ab7899).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab61700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
Peptide ELISA: antibody detection limit dilution 1:128,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. - Information by UniProt
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Database links
- Entrez Gene: 7072 Human
- Entrez Gene: 21841 Mouse
- Entrez Gene: 312510 Rat
- Omim: 603518 Human
- SwissProt: P31483 Human
- SwissProt: P52912 Mouse
- Unigene: 413123 Human
- Unigene: 333219 Mouse
see all -
Alternative names
- Cytotoxic granule associated RNA binding protein 1 antibody
- Cytotoxic granule associated RNA binding protein antibody
- mTIA-1 antibody
see all
Images
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Western blot - Anti-TIA1 antibody (ab61700)Anti-TIA1 antibody (ab61700) at 0.1 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 26 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lee Y et al. TIA1 variant drives myodegeneration in multisystem proteinopathy with SQSTM1 mutations. J Clin Invest 128:1164-1177 (2018). Read more (PubMed: 29457785) »
