Anti-TIA1 antibody (ab61700)
Key features and details
- Goat polyclonal to TIA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIA1 antibody
See all TIA1 primary antibodies -
Description
Goat polyclonal to TIA1 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-PKSTYESNTKQ
, corresponding to internal sequence amino acids 189-199 of Human TIA1 -
Positive control
- Jurkat whole cell lysate (ab7899).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab61700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. - Information by UniProt
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Database links
- Entrez Gene: 7072 Human
- Entrez Gene: 21841 Mouse
- Entrez Gene: 312510 Rat
- Omim: 603518 Human
- SwissProt: P31483 Human
- SwissProt: P52912 Mouse
- Unigene: 413123 Human
- Unigene: 333219 Mouse
see all -
Alternative names
- Cytotoxic granule associated RNA binding protein 1 antibody
- Cytotoxic granule associated RNA binding protein antibody
- mTIA-1 antibody
see all
Images
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Western blot - Anti-TIA1 antibody (ab61700)Anti-TIA1 antibody (ab61700) at 0.1 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 26 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
References (1)
ab61700 has been referenced in 1 publication.
- Lee Y et al. TIA1 variant drives myodegeneration in multisystem proteinopathy with SQSTM1 mutations. J Clin Invest 128:1164-1177 (2018). PubMed: 29457785
