• Product name

    Anti-TIA1 antibody - C-terminal
    See all TIA1 primary antibodies
  • Description

    Rabbit polyclonal to TIA1 - C-terminal
  • Host species

  • Specificity

    Publications demonstrate the importance of RNA-binding domains in determining cytoplasmic and nuclear localisation of TIA-1, and the assembly of stress granules in cells subjected to environmental stress.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human TIA1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab41475)

  • Positive control

    • WB: Jurkat whole cell and nuclear lysate. ICC/IF: HeLa, MCF7 cells. IHC-P: Human lymphoma tissue.



Our Abpromise guarantee covers the use of ab40693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function

    Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization

    Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytotoxic granule associated RNA binding protein 1 antibody
    • Cytotoxic granule associated RNA binding protein antibody
    • mTIA-1 antibody
    • Nucleolysin TIA 1 isoform p40 antibody
    • Nucleolysin TIA-1 isoform p40 antibody
    • Nucleolysin TIA1 isoform p40 antibody
    • p40 TIA 1 antibody
    • p40-TIA-1 (containing p15-TIA-1) antibody
    • p40-TIA-1 antibody
    • RNA binding protein TIA 1 antibody
    • RNA binding protein TIA1 antibody
    • RNA-binding protein TIA-1 antibody
    • T-cell-restricted intracellular antigen-1 antibody
    • TIA 1 antibody
    • TIA 1 cytotoxic granule associated RNA binding protein antibody
    • Tia antibody
    • TIA-1 antibody
    • TIA1 antibody
    • TIA1 cytotoxic granule associated RNA binding protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 protein antibody
    • TIA1_HUMAN antibody
    • TIAL1 antibody
    • TIAR antibody
    • WDM antibody
    see all


  • All lanes : Anti-TIA1 antibody - C-terminal (ab40693) at 1 µg/ml

    Lane 1 : Jurkat (human T cell lymphoblast-like cell line) whole cell lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 30 kDa, 58 kDa. We are unsure as to the identity of these extra bands.

    An additional band at 41 kDa is seen in Jurkat nuclear lysate. This possibly corresponds to TIAR, a closely related protein of TIA1, found mainly in the nucleus.

  • ab40693 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40693 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • ab40693 staining TIA1 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with  peroxidase  for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody.  The image shows clean predominantly nuclear staining.

    See Abreview


This product has been referenced in:

  • Mazloomian A  et al. Pharmacological systems analysis defines EIF4A3 functions in cell-cycle and RNA stress granule formation. Commun Biol 2:165 (2019). Read more (PubMed: 31069274) »
  • Apicco DJ  et al. Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo. Nat Neurosci 21:72-80 (2018). Read more (PubMed: 29273772) »
See all 11 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (Fibroblast)
Yes - 0.5 % Triton X-100
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 24 2019

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain)
Yes - TBS with 0.25% Triton
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Miss. Samantha Boudeau

Verified customer

Submitted Aug 18 2017


Thank you for submitting an Abreview of ab40693. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

- blocking longer (e.g. overnight)
- incubating the primary antibody for only 1-2 hours at RT

If you decide to follow these suggestions, please let me know if they are helpful.

This antibody is covered by our Abpromise, andifthe antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.
Please let me know your order or PO number in that regard.

I look forward to your reply.

Read More
Western blot
Human Cell lysate - whole cell (neuroblastoma)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (3-8% tris-acetate)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 17 2012


Thank you for your interest in our products.

I have blasted the immunogen against chicken and zebrafish. Chicken shows 74% identity and zebrafish shows no significant similarity.

According with these results we do not expect the antibody to recognise these species. We usually offer discount codes when the homology between both sequences is over 85%.

I am sorry I could not be more helpful. Please do not hesitate to contract us if you need further advice or information.

Read More
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Jul 14 2009

For licensing inquiries, please contact partnerships@abcam.com

Sign up