Product nameAnti-TIA1 antibody - C-terminal
See all TIA1 primary antibodies
DescriptionRabbit polyclonal to TIA1 - C-terminal
SpecificityPublications demonstrate the importance of RNA-binding domains in determining cytoplasmic and nuclear localisation of TIA-1, and the assembly of stress granules in cells subjected to environmental stress.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey
Synthetic peptide within Human TIA1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- WB: Jurkat whole cell and nuclear lysate. ICC/IF: HeLa, MCF7 cells. IHC-P: Human lymphoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab40693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionInvolved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationCytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
- Information by UniProt
- Cytotoxic granule associated RNA binding protein 1 antibody
- Cytotoxic granule associated RNA binding protein antibody
- mTIA-1 antibody
All lanes : Anti-TIA1 antibody - C-terminal (ab40693) at 1 µg/ml
Lane 1 : Jurkat (human T cell lymphoblast-like cell line) whole cell lysate
Lane 2 :
Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 30 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
An additional band at 41 kDa is seen in Jurkat nuclear lysate. This possibly corresponds to TIAR, a closely related protein of TIA1, found mainly in the nucleus.
ab40693 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40693 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
ab40693 staining TIA1 in human lymphoma tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with peroxidase for 5 minutes followed by a protein block for 10 minutes at 20°C . Antigen retrieval was by heat mediation in target retrieval solution. Samples were incubated with primary antibody 1/2000 for 45 minutes at 20°C. An HRP-conjugated Goat polycolonal to rabbit IgG was used as secondary antibody. The image shows clean predominantly nuclear staining.
This product has been referenced in:
- Apicco DJ et al. Reducing the RNA binding protein TIA1 protects against tau-mediated neurodegeneration in vivo. Nat Neurosci 21:72-80 (2018). Read more (PubMed: 29273772) »
- Jiang L et al. TIA1 regulates the generation and response to toxic tau oligomers. Acta Neuropathol N/A:N/A (2018). Read more (PubMed: 30465259) »