Overview

  • Product name
    Anti-TIA1 antibody [EPR9304]
    See all TIA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9304] to TIA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TIA1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HuT-78, Jurkat, Molt4, NIH/3T3 and K562 cell lysates. IHC-P: Human spleen tissue. ICC/IF: HuT-78 cells. IP: HuT-78 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/1000.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization
    Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytotoxic granule associated RNA binding protein 1 antibody
    • Cytotoxic granule associated RNA binding protein antibody
    • mTIA-1 antibody
    • Nucleolysin TIA 1 isoform p40 antibody
    • Nucleolysin TIA-1 isoform p40 antibody
    • Nucleolysin TIA1 isoform p40 antibody
    • p40 TIA 1 antibody
    • p40-TIA-1 (containing p15-TIA-1) antibody
    • p40-TIA-1 antibody
    • RNA binding protein TIA 1 antibody
    • RNA binding protein TIA1 antibody
    • RNA-binding protein TIA-1 antibody
    • T-cell-restricted intracellular antigen-1 antibody
    • TIA 1 antibody
    • TIA 1 cytotoxic granule associated RNA binding protein antibody
    • Tia antibody
    • TIA-1 antibody
    • TIA1 antibody
    • TIA1 cytotoxic granule associated RNA binding protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 protein antibody
    • TIA1_HUMAN antibody
    • TIAL1 antibody
    • TIAR antibody
    • WDM antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: TIA1 knockout HAP1 cell lysate (40 µg)
    Lane 3: Jurkat cell lysate (40 µg)
    Lane 4: K562 cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab140595 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab140595 was shown to specifically react with TIA1  when TIA1  knockout samples were used. Wild-type and TIA1  knockout samples were subjected to SDS-PAGE. Ab140595 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-TIA1 antibody [EPR9304] (ab140595) at 1/5000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42-43 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-TIA1 antibody [EPR9304] (ab140595) at 1/5000 dilution (purified)

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : HUT-78 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42-43 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with purified ab140595 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of HuT-78 cells labelling TIA1 with purified ab140595 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • ab140595 (purified) at 1/40 immunoprecipitating TIA1 in HuT-78 whole cell lysate.

    Lane 1 (input): HuT-78 whole cell lysate (10µg)

    Lane 2 (+): ab140595 + HuT-78 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab140595 in HuT-78 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-TIA1 antibody [EPR9304] (ab140595) (unpurified)

    Lane 1 : HuT-78 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Molt-4 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with unpurified ab140595 at a dilution of 1/100.

References

This product has been referenced in:
  • Ma X  et al. Hippo signaling promotes JNK-dependent cell migration. Proc Natl Acad Sci U S A 114:1934-1939 (2017). Read more (PubMed: 28174264) »
  • Hirsch-Reinshagen V  et al. Clinical and neuropathological features of ALS/FTD with TIA1 mutations. Acta Neuropathol Commun 5:96 (2017). IHC-P, ICC/IF ; Human . Read more (PubMed: 29216908) »
See all 3 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Feb 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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