Product nameTIA1 overexpression 293T lysate (whole cell)
ab94248 is a 293T cell transfected lysate in which Human TIA1 has been transiently over-expressed using a pCMV-TIA1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. Similarity: Contains 3 RRM (RNA recognition motif) domains.
Our Abpromise guarantee covers the use of ab94248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94248 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-TIA1 antibody (ab89602) at 1/500 dilution
Lane 1 :
TIA1 overexpression 293T lysate (whole cell) (ab94248)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94248 has not yet been referenced specifically in any publications.