Overview

  • Product name

  • Description

    Rabbit polyclonal to TIAL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 325-375 of Human TIAL1 (NP_003243.1).

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab129499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • Sequence similarities

    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aging associated gene 7 protein antibody
    • Nucleolysin TIAR antibody
    • T cluster binding protein antibody
    • TCBP antibody
    • TIA 1 related nucleolysin antibody
    • TIA-1-related protein antibody
    • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
    • TIA1 related protein antibody
    • TIAL1 antibody
    • TIAR antibody
    • TIAR_HUMAN antibody
    see all

Images

  • All lanes : Anti-TIAL1 antibody (ab129499) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 30 seconds
  • Detection of TIAL1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab129499 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Wang Q  et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). Read more (PubMed: 30569136) »
See 1 Publication for this product

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