Overview

  • Product name

  • Description

    Goat polyclonal to TIAL1
  • Host species

    Goat
  • Specificity

    ab179945 is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human TIAL1 aa 105-119 (internal sequence) (Cysteine residue). (NP_003243.1).
    Sequence:

    SPEITTEDIKSAFAP


    Database link: Q01085

  • Positive control

    • K562 cell lysate; Human skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab179945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling TIAL1 with ab179945 at 3.75 µg/ml.

  • Anti-TIAL1 antibody (ab179945) at 2 µg/ml + K562 cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab179945 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Ovary)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Ovary
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 08 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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