Key features and details
- Goat polyclonal to TIAL1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TIAL1 antibody
See all TIAL1 primary antibodies
DescriptionGoat polyclonal to TIAL1
Specificityab179945 is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Gorilla
- K562 cell lysate; Human skin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab179945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionRNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
modificationsPhosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes.
- Information by UniProt
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling TIAL1 with ab179945 at 3.75 µg/ml.
Anti-TIAL1 antibody (ab179945) at 2 µg/ml + K562 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab179945 has not yet been referenced specifically in any publications.