Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11323(B)] to TIAL1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TIAL1 antibody [EPR11323(B)]
    See all TIAL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11323(B)] to TIAL1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human TIAL1.

  • Positive control

    • WB: HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and ovarian carcinoma tissues. Flow Cyt: K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab169547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

    • Sequence similarities

      Contains 3 RRM (RNA recognition motif) domains.

    • Post-translational
      modifications

      Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Aging associated gene 7 protein antibody
      • Nucleolysin TIAR antibody
      • T cluster binding protein antibody
      • TCBP antibody
      • TIA 1 related nucleolysin antibody
      • TIA-1-related protein antibody
      • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
      • TIA1 related protein antibody
      • TIAL1 antibody
      • TIAR antibody
      • TIAR_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : TIAL1 knockout HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K-562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 41 kDa
      Observed band size: 49 kDa
      why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab169547 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab169547 Anti-TIAL1 antibody [EPR11323(B)] was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265793 (knockout cell lysate ab257743) was used.  Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE.  ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000  dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TIAL1 with ab169547 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TIAL1 with ab169547 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Flow Cytometry - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Flow cytometric analysis of permeabilized K562 cells labeling TIAL1 with ab169547 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
    • Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
      All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa

    References (0)

    Publishing research using ab169547? Please let us know so that we can cite the reference in this datasheet.

    ab169547 has not yet been referenced specifically in any publications.

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