Recombinant Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
![Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-393548-anti-tial1-antibody-epr11323-western-blot-human-knockout-lysate.jpg "Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11323(B)] to TIAL1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TIAL1 antibody [EPR11323(B)]
See all TIAL1 primary antibodies -
Description
Rabbit monoclonal [EPR11323(B)] to TIAL1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide corresponding to Human TIAL1.
-
Positive control
- WB: HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and ovarian carcinoma tissues. Flow Cyt (intra): K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11323(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 41 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
-
Database links
- Entrez Gene: 7073 Human
- Entrez Gene: 21843 Mouse
- Entrez Gene: 361655 Rat
- Omim: 603413 Human
- SwissProt: Q01085 Human
- SwissProt: P70318 Mouse
- Unigene: 501203 Human
- Unigene: 242072 Mouse
-
Alternative names
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
see all
Images
-
Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIAL1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab169547 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab169547 Anti-TIAL1 antibody [EPR11323(B)] was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265793 (knockout cell lysate ab257743) was used. Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE. ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-168198-anti-tial1-antibody-epr11323b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-168199-anti-tial1-antibody-epr11323b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-168197-anti-tial1-antibody-epr11323b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)Intracellular flow cytometric analysis of permeabilized K562 cells labeling TIAL1 with ab169547 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
-
![Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-168200-anti-tial1-antibody-epr11323b-western-blot.jpg)
Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
![Anti-TIAL1 antibody [EPR11323(B)] (ab169547)](https://www.abcam.com/ps/products/169/ab169547/Images/ab169547-7-benefits-of-recombinant-antibodies.png)
Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab169547 has not yet been referenced specifically in any publications.
