Product nameAnti-TIAL1 antibody [EPR11323(B)]
See all TIAL1 primary antibodies
DescriptionRabbit monoclonal [EPR11323(B)] to TIAL1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human TIAL1 (UniProt Q01085).
- HeLa, Jurkat and K562 cell lysates; Human breast and ovarian carcinoma tissues; K562 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 41 kDa.|
|IHC-P||1/50 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
modificationsPhosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes.
- Information by UniProt
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling TIAL1 with ab169547 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
ab169547 has not yet been referenced specifically in any publications.