Anti-TIAL1 antibody [EPR11323(B)] Recombinant (ab169547)

Product name

Anti-TIAL1 antibody [EPR11323(B)]
See all TIAL1 primary antibodies
Description

Rabbit monoclonal [EPR11323(B)] to TIAL1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide corresponding to residues in Human TIAL1 (UniProt Q01085).
Positive control
- HeLa, Jurkat and K562 cell lysates; Human breast and ovarian carcinoma tissues; K562 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11323(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab169547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
IHC-P		1/50 - 1/100.
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/1000 - 1/5000. Predicted molecular weight: 41 kDa.

IHC-P

1/50 - 1/100.

Flow Cyt

1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC or IP.

Function

RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Sequence similarities

Contains 3 RRM (RNA recognition motif) domains.
Post-translational
modifications

Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA.
Cellular localization

Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes.
Target information above from: UniProt accession Q01085 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7073 Human
- Entrez Gene: 21843 Mouse
- Entrez Gene: 361655 Rat
- Omim: 603413 Human
- SwissProt: Q01085 Human
- SwissProt: P70318 Mouse
- Unigene: 501203 Human
- Unigene: 242072 Mouse
- Unigene: 162475 Rat
see all
Alternative names
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
- TCBP antibody
- TIA 1 related nucleolysin antibody
- TIA-1-related protein antibody
- TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
- TIA1 related protein antibody
- TIAL1 antibody
- TIAR antibody
- TIAR_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Flow Cytometry - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

Flow cytometric analysis of permeabilized K562 cells labeling TIAL1 with ab169547 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Western blot - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)

All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

ab169547 has not yet been referenced specifically in any publications.

Publishing research using ab169547? Please let us know so that we can cite the reference in this datasheet.

Anti-TIAL1 antibody [EPR11323(B)] (ab169547)