Recombinant Anti-TIAM2 antibody [EPR16838] (ab199426)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16838] to TIAM2
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TIAM2 antibody [EPR16838]
See all TIAM2 primary antibodies -
Description
Rabbit monoclonal [EPR16838] to TIAM2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, 293, RAW 264.7, PC-12, NIH/3T3 and C6 whole cell lysates and human fetal brain tissue lysate. IHC-P: Human gliocytoma. ICC/IF: RAW 264.7. Flow Cyt (intra): K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16838 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/450.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/1000.
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Notes |
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Flow Cyt (Intra)
1/450. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
Target
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Function
Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA. -
Tissue specificity
Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis. -
Sequence similarities
Belongs to the TIAM family.
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains.
Contains 1 RBD (Ras-binding) domain. -
Domain
The PH 1 domain and amino acids 621-782 (a region called TSS; otherwise known as CC-Ex) are necessary for membrane localization. The PH 1 and TSS domains are necessary for Rac1 activity. The PH 2 domain is engaged in the enhancement of the catalytic activity of the adjacent DH domain. The PH 1, TSS and DH domains are necessary to induce neurite-like structure. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth. -
Cellular localization
Cytoplasm. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Localizes to the plasma membrane in neurites. - Information by UniProt
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Database links
- Entrez Gene: 26230 Human
- Entrez Gene: 24001 Mouse
- Entrez Gene: 100362710 Rat
- Omim: 604709 Human
- SwissProt: Q8IVF5 Human
- SwissProt: Q6ZPF3 Mouse
- Unigene: 586279 Human
- Unigene: 137134 Mouse
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Alternative names
- FLJ41865 antibody
- OTTHUMP00000017472 antibody
- SIF and TIAM1 like exchange factor antibody
see all
Images
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All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/10000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : 293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used only secondary antibody.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of Raw264.7 cells labelling TIAM2 with ab199426 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow)cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.
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Anti-TIAM2 antibody [EPR16838] (ab199426) at 2000 cells + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution
Lane 1 : Rat brain lysate at 10 µg
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 mg/ml
Lane 4 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
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Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution + C6 (rat glial tumor cells) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab199426 has not yet been referenced specifically in any publications.