Anti-TICAM2 antibody (ab227271)
Key features and details
- Rabbit polyclonal to TICAM2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TICAM2 antibody
See all TICAM2 primary antibodies -
Description
Rabbit polyclonal to TICAM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human TICAM2.
Database link: Q86XR7 -
Positive control
- WB: HCT 166 whole cell lysate. IHC-P: Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 27 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. -
Tissue specificity
Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas. -
Sequence similarities
Contains 1 TIR domain. -
Domain
The TIR domain mediates the interaction with TRAF6. -
Post-translational
modificationsPhosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling. -
Cellular localization
Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. - Information by UniProt
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Database links
- Entrez Gene: 100302736 Human
- Entrez Gene: 353376 Human
- Omim: 608321 Human
- SwissProt: Q86XR7 Human
- Unigene: 709120 Human
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Alternative names
- Putative NF-kappa-B-activating protein 502 antibody
- TCAM2_HUMAN antibody
- TICAM-2 antibody
see all
Images
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Western blot - Anti-TICAM2 antibody (ab227271)Anti-TICAM2 antibody (ab227271) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 27 kDa12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TICAM2 antibody (ab227271)Paraffin-embedded human colon carcinoma tissue stained for TICAM2 with ab227271 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227271 has not yet been referenced specifically in any publications.
