Anti-TICAM2 antibody (ab227271)

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Overview

  • Product name

    Anti-TICAM2 antibody
    See all TICAM2 primary antibodies

  • Description

    Rabbit polyclonal to TICAM2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey

  • Immunogen

    Recombinant full length protein corresponding to Human TICAM2.
    Database link: Q86XR7

  • Positive control

    • WB: HCT 166 whole cell lysate. IHC-P: Human colon carcinoma tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1.

  • Tissue specificity

    Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas.

  • Sequence similarities

    Contains 1 TIR domain.

  • Domain

    The TIR domain mediates the interaction with TRAF6.

  • Post-translational
    modifications

    Phosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
    Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.

  • Cellular localization

    Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Putative NF-kappa-B-activating protein 502 antibody
      • TCAM2_HUMAN antibody
      • TICAM-2 antibody
      • TICAM2 antibody
      • TIR containing adapter molecule 2 antibody
      • TIR domain containing adaptor protein antibody
      • TIR domain-containing adapter molecule 2 antibody
      • TIRAP 3 antibody
      • TIRAP3 antibody
      • TIRAP3a short form antibody
      • TIRP antibody
      • Toll-like receptor adaptor protein 3 antibody
      • Toll/interleukin-1 receptor domain-containing protein antibody
      • TRAM antibody
      • TRIF-related adapter molecule antibody
      see all

    Images

    • Western blot - Anti-TICAM2 antibody (ab227271)
      Western blot - Anti-TICAM2 antibody (ab227271)
      Anti-TICAM2 antibody (ab227271) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

      Developed using the ECL technique.

      Predicted band size: 27 kDa



      12% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TICAM2 antibody (ab227271)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TICAM2 antibody (ab227271)

      Paraffin-embedded human colon carcinoma tissue stained for TICAM2 with ab227271 at 1/500 dilution in immunohistochemical analysis.

    References

    ab227271 has not yet been referenced specifically in any publications.

    Publishing research using ab227271? Please let us know so that we can cite the reference in this datasheet.

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